3种广西钩藤属药用植物的RAPD多态性分析

目的运用随机扩增多态性技术探索广西钩藤属药用植物的遗传多态性,为其种质鉴定提供依据。方法采用优化的试剂盒提取法提取大叶钩藤、白钩藤、毛钩藤3种植物的总DNA。用经过筛选的18条10个碱基的随机引物进行PCR扩增。结果优化后的试剂盒提取法可有效获取3种钩藤属植物的基因组DNA,18条随机引物扩增得到条带共260条,其中多态性条带231条,多态率达到88.9%,扩增效果好。按UPGMA法进行聚类分析,计算其遗传相似系数,结果显示,5份钩藤供试材料聚为3类。结论 3种广西钩藤属药用植物的聚类结果与其种和地理区域远近一致,与形态学观察可能存在差异。所得RAPD标记可用于钩藤属药用植物的多态性研究,并为今后开发遗传鉴定的分子标记奠定基础。

Polymorphism of Three Kinds of Medicinal Plants Uncaria from Guangxi by RAPD

Objective To research the polymorphism of three kinds of medicinal plants Uncaria.from Guangxi,by radom amplified polymorphic DNA(RAPD) analysis and provide a basis for germplasm identification. Methods The modified extraction kit was used to extract the genomic DNA of several plants,including 3 regions of Uncaria Macrophylla Wall,Uncaria Sessilifmctus Roxb.and Uncaria Hirsute Havil,then amplified by 18 selected random primers with 10bp. Results Optimized extraction kit was effective for 3 Uncaria species genome DNA.With the DNA extracted from several plants as template,18 primers amplified a total of 260 bands,231 bands were polymorphic,polymorphism rate was 88.85%,and the effect amplification was good.Then we constructed a dendrogram based on genetic distances by using UPGMA method.The dendogram reflected that 5 samples clustered into three taxa. Conclusion This result is in accordance with their species and geographic origin,but may be different from morphological observed.RAPD markers can be used to analyze polymorphisms of medicinal plants Uncaria,and provide the genetic basis for identification.