和厚朴酚与青蒿素体外抗人胃癌MGC-803细胞的作用研究

目的考察和厚朴酚、青蒿素抗肿瘤及两者联合用药的效果。方法采用MTT测定和厚朴酚、青蒿素对人胃癌MGC-803细胞、正常肝细胞LO2抑制作用,用IC50评测体外直接抗肿瘤效果,用金氏公式进行联合用药分析。结果 20μg/ml的和厚朴酚对人胃癌MGC-803细胞具有快速而不可逆的杀伤作用;和厚朴酚能抑制人胃癌MGC-803细胞、正常肝细胞LO2的增殖,IC50分别为3.96,8.04μg/ml。青蒿素对其没有明显的抑制作用,IC50分别为64.85、93.92μg/ml。3.5μg/ml的和厚朴酚与12.5,10,7.5μg/ml的青蒿素联合应用对人胃癌MGC-803细胞体外抑制呈相加作用,Q值分别为1.05,1.04,1.12;与30,20,15μg/ml青蒿素联合应用对人胃癌MGC-803细胞体外抑制呈协同作用,Q值分别为1.21,1.31,1.24;2μg/ml和厚朴酚与30,20,15,12.5,10,7.5μg/ml青蒿素联合应用对人胃癌MGC-803细胞体外抑制呈协同作用Q值分别为1.27,1.28,1.32,1.39,1.16,1.25。结论和厚朴酚对MGC-803细胞较强的抑制肿瘤生长的作用;青蒿素对人胃癌MGC-803细胞、正常肝细胞LO2的毒性低,和厚朴酚与青蒿素联合应用对人胃癌MGC-803细胞可产生协同作用或相加作用。

Anti - tumor Effect of Honokiol and its Combination with Artemsinin on MGC - 803 Cells

Objective To observe the anti-tumor effects of honokiol and artemisinin on MGC-803 cells,LO2 cells and the combination effect of honokiol minbea with artemisininin. Methods MTT assay was used to detect the anti-tumor effects of honokiol and artemisinin on MGC-803cells,IC50 was used to measure the direct anti-tumor effect and King formula was applied to analyze the combination effect of the drugs. Results Treatment with honokiol at the concentration of 20 μg/ml for a short time could inhibit irrevesibly the growth of MGC-803 cells.Honokiol could inhibit the proliferation of MGC-803 and LO2 cells,the IC50 was 3.96,8.04 μg/ml,respective,but the artemisinin had no obviously inhibitory effect on MGC-803 cells and LO2 cells,the IC50 was 64.85,93.92 μg/ml,respectively.Honokiol at the concentrations of 3.5 μg/ml with 12.5,10,7.5 μg/ml of artemisinin showed additive effects on MGC-803 cells,the Q were 1.05,1.04,1.12 respectively.Honokiol at the concentrations of 3.5 μg/ml with 30,20,15 μg/ml of artemisinin showed synergistc effects and the Q were1.21,1.31,1.24,respectively.Honokiol at the concentrations of 2 μg/ml with 30,20,15,12.5,10,7.5 μg/ml of artemisinin showed synergistc effects on MGC-803 cells and the Q values were 1.27,1.28,1.32,1.39,1.16,1.25 respectively. Conclusion Honokiol has obvious anti-tumor effect on MGC-803 cells.Artemisinin has no obviously cytotoxicity to MGC-803 cells and human normal liver LO2 cells.Honokiol combined with artemisinin has synergistc or additive effect on MGC-803 cells.