| 桑叶多糖的提取纯化及其含量测定 |
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| 引用本文： | 欧阳臻,常钰,李永辉,陈钧.桑叶多糖的提取纯化及其含量测定[J].时珍国医国药,2005,16(10):961-962. |
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| 作者姓名： | 欧阳臻 常钰 李永辉 陈钧 |
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| 作者单位： | 江苏大学生物与环境工程学院制药工程系,江苏,镇江,212013 |
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| 基金项目： | 江苏省科技攻关基金项目（No.BE2004352） |
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| 摘 要： | 目的：纯化桑叶多糖并测定其含量。方法：采用10％三氯乙酸、大孔树脂初步纯化桑叶多糖，采用硫酸-蒽酮法对其含量进行测定。结果：用三氯乙酸除蛋白的方法是可行的；桑叶中多糖的平均含量为2．62％，平均回收率为98．81％，RSD为2．08％。结论：该法可作为桑叶中多糖的含量测定方法。
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| 关 键 词： | 桑叶多糖 提取纯化 硫酸-蒽酮法 |
| 文章编号： | 1008-0805（2005）10-0961-02 |
| 收稿时间： | 2005-01-18 |
| 修稿时间： | 2005-06-21 |
Purification and Determination of Polysaccharide in Morus alba L. |
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| Ouyang Zhen,CHANG Yu,LI Yong-hui,CHEN Jun.Purification and Determination of Polysaccharide in Morus alba L.[J].Lishizhen Medicine and Materia Medica Research,2005,16(10):961-962. |
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| Authors: | Ouyang Zhen CHANG Yu LI Yong-hui CHEN Jun |
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| Institution: | Parmaceutical Engineering Dep. of Biology and Environment Engineering School, Jiangsu University, Zhenjiang, Jiangsu 212013, China |
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| Abstract: | Objective:To purify and determine the contents of polysaccharide in Morus alba L.Methods:The polysaccharide were preliminarily purified by 10% trichloroacetic acid and resin.The contents of polysaccharide were determined by sulfuric acid anthrone method and detected at 620 nm by UV/Visible spectrophotometry.Results:The average contents of polysaccharide in Morus alba L.was 2.62%.The recovery rate was 98.81% with 2.08% of RSD(n=5).Conclusion:The sulfuric acid anthrone method is reasonable. |
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| Keywords: | Morus alba L Polysaccharide Purification Sulfuric acid anthrone |
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