玄参提取物抗炎与抗动脉硬化作用的探索

目的 观察玄参提取物对大鼠动脉硬化及其炎症相关细胞因子的影响.方法 将大鼠随机分为正常组、模型组、玄参提取物低剂量组、玄参提取物高剂量组和布洛芬组.模型组及各给药组采用一次性腹腔注射维生素D_3 60万单位/kg,同时每天灌服10 ml/kg脂肪乳的方法制备大鼠动脉硬化模型.玄参低剂量组和高剂量组分别每天灌服玄参提取物20.5 mg·kg~(-1)·d~(-1)和41.0 mg·kg~(-1).D~(-1),布洛芬组每天灌服布洛芬混悬液144 mg·kg~(-1)·d~(-1),正常组和模型组每天灌服等量蒸馏水.给药70 d后处死大鼠,对血脂、TNF-α,IL-1β,IL-6,IL-10 及NF-кB进行测定,并观察动脉的病变情况.结果 玄参提取物可明显降低模型大鼠胆固醇和低密度脂蛋白的水平,提高高密度脂蛋白与低密度脂蛋白的比值,降低炎症因子TNF-α,IL-1β,IL-6的浓度,提高抗炎因子IL-10的浓度,抑制动脉NF-кB过量表达,抑制模型大鼠动脉壁中膜的增厚.结论 玄参提取物可通过抗炎和降血脂而发挥一定的抗动脉硬化的作用.

Research on the Anti-inflammation and Anti-atherosclerosis of Extracts from Radix Scrophulariae

Objective To study the effect of extract from Radix Scrophulariae (RS) against atherosclerosis and the relative mechanisms in rats.Methods Rats were randomly divided into 5 groups. They were the conrtol , model,low and high dose of RS extract and ibuprofen groups.The model rats were intraperitoneally injected with 600 000 IU·kg-1 vitamine D_3 and intragastrically administrated high-fat emulsion 10 ml· kg~(-1)·d~(-1) for 70 days.At the same time, the model rats treated with drugs were orally given 20.5mg·kg~(-1).D~(-1),41.0 mg·kg~(-1)·d~(-1) RS extract and 144.0 mg·kg~(-1)·d~(-1) ibuprofen suspension respectively.After 70days,the rats were killed, and the serum levels of TC,TG,LDL,HDL/LDL,TNF-α,IL-1β,IL-6,IL-10,the expression of NF-кB in rat aorta and the pathological change of aorta were measured.Results RS extract could lower the serum levels of TC,LDL-C, TNF-α,IL-1β and IL-6, increase the level of IL-10 and the ratio of HDL/LDL,inhibit the expression of NF-кB and reduce the thickness of the artery wall.Conclusion RS extract has anti-atherescle ratic effect.The mechanism may be related with inhibiting inflammation and lowering serum lipid.