刺五加多糖对过氧化氢诱导海马神经元氧化应激损伤及细胞凋亡相关基因-2家族的影响

目的观察刺五加多糖(ASPS)对H2O2诱导海马神经元氧化应激损伤及细胞凋亡相关基因-2(Bcl-2)家族的影响,并探讨其可能机制。方法运用海马神经细胞原代培养技术,采用H2O2诱导建立细胞氧化损伤模型。观察细胞形态学变化;MTT法测定细胞活性;TUNEL法,检测H2O2诱导大鼠海马神经元凋亡;免疫组化法检测细胞中Bc l-2,Bax蛋白表达;化学比色法检测细胞匀浆液中一氧化氮合酶(NOS),过氧化物歧化酶(SOD)的活性及丙二醛(MDA)含量。结果形态学观察显示:与模型组比较,ASPS组细胞损伤程度明显减轻;ASPS组细胞活性比模型组细胞活性高;TUNEL法显示ASPS预处理组细胞阳性率明显高于模型组细胞;ASPS组Bcl-2表达量较高而Bax表达量较低;NOS活力及MDA生成量ASPS组明显低于模型组,但SOD活力ASPS组显著高于模型组。结论ASPS能提高海马神经细胞的抗氧化应激损伤能力并上调Bcl-2基因表达,下调Bax表达。

Effects of Acanthopanacis senticosi Polysaccharides on Oxidative Stress Damage and Bcl-2 Family in Hippocampal Neurons Impaired by H2O2

Objective To observe the effects of Acanthopanacis senticosi polysaccharides on oxidative stress damage and Bcl-2 family in hippocampal neurons impaired by H2O2 and to reveal the possible mechanism of this effect.Methods The model of oxidative damage was induced by H2O2.The change of cell morphology was observed;the viability of hippocampal neurons was detected with MTT method;apoptosis in hippocampal neurons induced by H2O2 was detected with TUNEL method,the expression of Bcl-2 and Bax were detected by imm...