| 西黄丸含药血清对人乳腺癌细胞生长的影响 |
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| 引用本文： | 梁文波,张学梅,宋旦旨. 西黄丸含药血清对人乳腺癌细胞生长的影响[J]. 时珍国医国药, 2007, 18(6): 1371-1372 |
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| 作者姓名： | 梁文波 张学梅 宋旦旨 |
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| 作者单位： | 大连大学医学院,辽宁,大连,116622 |
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| 摘 要： | 目的研究西黄丸含药血清对人乳腺癌细胞系(MCF-7)生长及其细胞周期分布的影响。方法应用MTT比色法检测肿瘤细胞活性,流式细胞仪分析细胞周期相对分布。结果西黄丸含药血清对人乳腺癌细胞系(MCF-7)有较强的抑制作用,其抑制率与含药血清浓度呈直线相关。流式细胞仪周期分析结果显示含药血清处理24 h,S期细胞比例增加,G2/M期细胞减少;处理48 h后,G0/1期细胞增多,S期细胞减少。结论西黄丸含药血清可抑制人乳癌细胞系(MCF-7)细胞生长,并可干扰其细胞周期。
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| 关 键 词： | 西黄丸含药血清 人乳腺癌细胞 细胞周期 |
| 文章编号： | 1008-0805（2007）06-1371-01 |
| 收稿时间： | 2006-12-21 |
| 修稿时间： | 2006-12-212007-03-05 |
The Effects of Xihuang Pill Drug Serum on the Growth and Cell Cycle of Human Breast Carcinoma Cells |
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| LIANG Wen-bo,ZHANG Xue-mei,SONG Dan-zhi. The Effects of Xihuang Pill Drug Serum on the Growth and Cell Cycle of Human Breast Carcinoma Cells[J]. Lishizhen Medicine and Materia Medica Research, 2007, 18(6): 1371-1372 |
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| Authors: | LIANG Wen-bo ZHANG Xue-mei SONG Dan-zhi |
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| Affiliation: | College of Medicine, Dalian University , Dalian, 116622, China |
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| Abstract: | Objective To investigate the effects of Xihuang pill drug serum on the growth of breast cell(MCF-7) and the cell cycle.Methods Activity of tumor cell was detected by MTT and cell cycle was analyzed by flow cytometry.Results Xihuang pill drug serum had an inhibitory effect on the rumor cell,which was positively related to the drug serum content.Typital morphological changes of apoptosis were observed under the transmission electron microscope.FCM showed that the cell cycle treated with Xihuang pill drug serum for 24 h,the ratio of cells in S phase increased,while that in G2/M phase decreased;after 48 h treatment,the ratio of cells in G0/1phase increased,and S phase decreased.Conclusion Xihuang pill drug serum can inhibit the growth of breast carcinoma cell(MCF-7) and its cell cycle in vitro. |
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| Keywords: | Xihuang pill drug serum Human breast carcinoma cells Cell cycle |
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