| 广佛手rDNA ITS序列测定及特点的初步分析 |
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| 引用本文： | 高晓霞,陈晓颖,罗源生.广佛手rDNA ITS序列测定及特点的初步分析[J].时珍国医国药,2007,18(1):162-163. |
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| 作者姓名： | 高晓霞 陈晓颖 罗源生 |
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| 作者单位： | 1. 广东药学院,广东,广州,510006
2. 广东广弘药材有限公司,广东,广州,510089 |
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| 摘 要： | 目的分析广佛手及其近缘种香橼的rDNA ITS序列及其规律。方法采用PCR直接测序技术测定广佛手和香橼rDNA ITS序列并作序列变异分析。结果4个植物样品rDNA ITS序列长度为644-672bp。其中5．8S rDNA长度为165bp，编码区较保守。广佛手和香橼的ITS1和ITS2序列平均遗传距离分别为0．0095和0．0142。结论rDNA ITS序列可为鉴定佛手提供分子参照系统。
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| 关 键 词： | 佛手 香橼 rDNA ITS序列 |
| 文章编号： | 1008-0805(2007)01-0162-02 |
| 修稿时间： | 2006年5月8日 |
Preliminary Study on rDNA ITS Sequencing and Characteristics of Citrus medica L. var. sarcodactylis ( Noot. ) Swingle |
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| GAO Xiao-xia,CHEN Xiao-ying,LUO Yuan-sheng.Preliminary Study on rDNA ITS Sequencing and Characteristics of Citrus medica L. var. sarcodactylis ( Noot. ) Swingle[J].Lishizhen Medicine and Materia Medica Research,2007,18(1):162-163. |
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| Authors: | GAO Xiao-xia CHEN Xiao-ying LUO Yuan-sheng |
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| Abstract: | Objective The ribosomal DNA internao transcribed spacers(ITS)and 5.8S rDNA ITS sequence of Citrus medica L.var.sarcodactylis(Nootc).Swingle and C.medicaL.were sequenced.Methods The rDNA ITS gene fragment was amplified by PCR with universal primers of rDNA ITS and sequenced.Results The DNA sequence of ITS ranged from 644 bp to 672 bp and the(5.8)S gene of all of the samples was 165 bp in size.Distance values of ITS1 and ITS2 sequence,estimated according to Kimura two-parameter models between Citrus medicaL.var.sarcodactylis(Noot.)Swingle and its adulterant and C.medica L.,were 0.0095 and 0.0142,respectively.Conclusion rDNA ITS sequence may be the evidence for the molecular authentication of Citrus medica L.var.sarcodactylis(Noot.)Swingle. |
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| Keywords: | Citrus medica L var sarcodactyis(Noot ) Swingle C medica L rDNA internal transcribed spacers sequence |
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