针刺捻转补泻法对血虚证模型大鼠血红蛋白、血清铁蛋白等的影响

目的观察针刺足三里捻转补泻手法对血虚证大鼠Hb（Hematoglobin,血红蛋白）、SF（Serum ferritin,血清铁蛋白）等的影响,探讨针刺捻转补泻手法与效应的关系。方法将176只SD大鼠随机分为5组：空白组（10只）、模型对照组（46只）、针刺组（40只,不做补泻手法）、捻转补法组（40只）和捻转泻法组（40只）,后3组统称为治疗组。模型对照组和治疗组采用放血加限食法制作血虚证模型,隔天治疗1次,共10次。针刺开始后,分别于第6、11、16、21天采血,检测Hb、SF和血清ATP（Adenosine TriPhosphate,三磷酸腺苷）酶活性。结果针刺后第6天各治疗组大鼠Hb、SF高于模型对照组（P〈0.05）;针刺后第11天,捻转补法组和捻转泻法组大鼠各项指标均高于模型对照组（P〈0.05）;针刺后第16天的SF、针刺后第6、11天的血清ATP酶活性,捻转补法组高于捻转泻法组（P〈0.05）。结论①单纯针刺、捻转补法、捻转泻法对血虚证大鼠的恢复均有治疗作用;②在血虚证的状态下,捻转补法的疗效要优于捻转泻法。

Effect of Twisting-twirling Acupuncture Reinforcing and Reducing Method on Hb and SF in Blood Deficiency Rats

Objective To observe the effect of acupuncturing with twisting-twirling reinforcing and reducing method on Hb, SF and the activity of ATPase in blood-deficiency syndrome rats and to study the relationship between twisting-twirling reinforcement and reduction method and its effect. Method 176 rats were divided into five groups randomly： blank group （10）, model group （46）, quiet stay needle group （40）, reinforcing group （40） and reducing group （40）. the last three groups were called as treating groups. Blood deficiency model were made on model group and treating grgups by bleeding and food restriction. Do treatment every other day,10 times totally. Measure Hb, SF and the activity of ATpase respectively after 6, 1l, 16, 21 days treatment. Result After 6 days treatment, the Hb and SF of treating group were obviously higher than those of modet group （P〈0 05）; After 11 clays treatment, the Hb, SF and the activity of ATPase of the reinforcing group and the reducing group were obviously higher than those of model group（P 〈0.05）; After 16 days treatment, the SF of the reinforcing group were higher than those ofthe reducing group （P〈0.05）; After 6 11 days treatment, the activity of ATPase of the reinforcing group were higher than those of the reducing group （P〈0.05）. Conclusion All these treatment are useful for the recovery of blood deficiency rats. for the blood-deficiency syndrome rats, the reinforcement by left twirling treatment is much better than the reduction by right twirling treatment.