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高效液相色谱法测定银胡感冒海绵剂中绿原酸的含量
引用本文:陈锋,姜平川,李嘉.高效液相色谱法测定银胡感冒海绵剂中绿原酸的含量[J].中国民族民间医药杂志,2010,19(7):28-29.
作者姓名:陈锋  姜平川  李嘉
作者单位:广西中医药研究院;
摘    要:目的:建立测定银胡感冒海棉剂中绿原酸含量的高效液相色谱方法。方法:采用Global Chromatography C18柱,以甲醇-0.4%磷酸(25:75)为流动相,流速1ml/min,检测波长327nm,柱温30℃。结果:绿原酸在0.152~0.76μg范围内,有良好的线性关系,回归方程为Y=31.4334x-12.7125,r=0.9999;平均回收率为99.69%,RSD=2.43%(n=6)。结论:该所建方法简便,快捷,重复性均较好,可用于银胡感冒海绵剂的质量控制。

关 键 词:高效液相色谱法  银胡感冒海绵剂  绿原酸

Determination of Chlorogenic Acid in the Yinhu Gaomao San by HPLC
CHEN Feng,JIANG Ping-chuan,LI Jia.Determination of Chlorogenic Acid in the Yinhu Gaomao San by HPLC[J].Chinese Journal of Ethnomedicine and Ethnopharmacy,2010,19(7):28-29.
Authors:CHEN Feng  JIANG Ping-chuan  LI Jia
Institution:( Guangxi Insititute of Chinese Medicine & Pharmaceutical Science, Guangxi, Manning, 530022, China)
Abstract:Objetctive: To establish a method for the determination of chlorogenic acid in the Yinhu Gaomao Haimianji. Methods: The chromatographic conditions were as fotlows: The separation was carried out on a Global Chromatography C18 column, the mobile phase consisted of methanol-0. 4% H3PO4 (25: 75), the detection wavelength at 327 nm and the flow rate being 1 ml · min^-1. The column temperature was 30℃. Results: A linearity of Chlorogenic acid in the extract was obtained in the range of 0. 152 - 0. 76μg , r = 0. 9999, The average recovery was 99. 69% and RSD = 2. 43%. Conclusion: The method is siMple, rapid, reliabl and can be used to control the quality of Chlorogenic acid in the Yinhu Gaomao San.
Keywords:HPLC  Chlorogenic acid  Yinhu Gaomao Haimianji  
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