引种不同来源穿心莲叶中穿心莲内酯及脱水穿心莲内酯积累分析

目的观察不同来源穿心莲叶中穿心莲内酯和脱水穿心莲内酯的积累,以期为品种选育提供依据。方法收集同一引种地各生长阶段的不同来源地穿心莲叶,以HPLC测定叶中穿心莲内酯和脱水穿心莲内酯的含量。结果经数据分析,穿心莲内酯和脱水穿心莲内酯与生长期呈现明显的相关性。穿心莲内酯含量无论大叶型还是小叶型与始花期均成正相关,即开花期越晚,生长期越长,含量越高,大叶型相关系数的平均值为0.951,小叶型为0.899;脱水穿心莲内酯含量与始花期均成负相关,即开花期越晚,脱水穿心莲内酯含量越低,大叶型相关系数均值为-0.930,小叶型为-0.979。结论建议穿心莲品种应从大叶型中选育始花期晚的品种。

Analysis on Accumulation of Andrographolide and 14-deoxy-11,12-didehy-droandrographolide in the Leaves of Andrographis paniculata Nees Introduced from Different Sources

Objective To observe the accumulation of andrographolide and 14-deoxy-11,12-didehy-droandrographolide in the leaves of Andrographis paniculata Nees introduced from different sources,and to obtain evidences for seed breeding of Andrographis paniculata Nees.Methods Leaves of Andrographis paniculata Nees at various growth stage planted in the same land as well as those came from different sources were collected,and andrographolide and 14-deoxy-11,12-didehy-droandrographolide in the leaves were determined by HPLC.Results The results of data analysis showed the contents of andrographolide and 14-deoxy-11,12-didehy-droandrographolide obviously correlated with the growing period.For either small-leaf type or large-leave type of Andrographis paniculata Nees,their andrographolide content had a positive correlation with the blooming date,i.e.the later the blooming date and the longer the growing period,the more the andrographolide content,and the correlation coefficient of large-leaf type was 0.951 and that of small-leaf type was 0.899.However,the content of 14-deoxy-11,12-didehy-droandrographolide was reverse,i.e.,the later the blooming date,the less the content,and the correlation coefficient of large-leaf type was-0.930 and that of small-leaf type was-0.979.Conclusion It is suggested that seed breeding of Andrographis paniculata Nees should select the large-leaf type with later blooming date.