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| 川楝子提取物体外抗单纯疱疹病毒1型活性的实验研究 | |
| 引用本文： | 赖志才,瞿畅,曾恕芬,裴赢,向阳飞,钱垂文,任哲,王一飞,张颖君,杨崇仁.川楝子提取物体外抗单纯疱疹病毒1型活性的实验研究[J].中药新药与临床药理,2010,21(1). |
| 作者姓名： | 赖志才 瞿畅 曾恕芬 裴赢 向阳飞 钱垂文 任哲 王一飞 张颖君 杨崇仁 |
| 作者单位： | 1. 暨南大学药学院,广州,510632;暨南大学生物医药研究开发基地,广州,510632 2. 中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,昆明,650204 3. 暨南大学生物医药研究开发基地,广州,510632 |
| 基金项目： | 国家自然科学基金·广东省人民政府自然科学联合基金，植物化学与西部植物资源持续利用国家重点实验室自主课题，开放课题 |
| 摘 要： | 目的在体外研究川楝子提取物抗单纯疱疹病毒1型(HSV-1)的作用。方法以Vero细胞为宿主细胞,阿昔洛韦为阳性对照药物,通过观察细胞病变效应(CPE)与空斑减数实验测定川楝子提取物抗HSV-1活性,计算其IC50与治疗指数,并从药物对病毒的直接灭活作用、对病毒吸附的影响及对病毒穿膜的影响三个方面初探川楝子提取物抗HSV-1活性的机理。结果川楝子提取物能明显抑制HSV-1的致病变作用,其IC50为18.26μg/mL,TI值为18。研究显示,川楝子提取物在体外对HSV-1直接灭活的效果明显,而对HSV-1吸附与穿入细胞的抑制作用较差。结论川楝子提取物在体外有明显的抗HSV-1感染作用,且主要是通过直接灭活HSV-1而发挥作用。 |
| 关 键 词： | 川楝子提取物 MTT法 细胞病变效应法(CPE) 空斑减数实验 |
Anti-HSV-1 Activity of the Extract from Fructus Toosendan In-vitro |
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| LAI Zhicai,QU Chang,ZENG Shufen,PEI Ying,XIANG Yangfei,QIAN Chuiwen,REN Zhe,WANG Yifei,ZHANG Yingjun,YANG Chongren.Anti-HSV-1 Activity of the Extract from Fructus Toosendan In-vitro[J].Traditional Chinese Drug Research & Clinical Pharmacology,2010,21(1). | |
| Authors: | LAI Zhicai QU Chang ZENG Shufen PEI Ying XIANG Yangfei QIAN Chuiwen REN Zhe WANG Yifei ZHANG Yingjun YANG Chongren |
| Institution: | LAI Zhicai1,2,QU Chang1,ZENG Shufen3,PEI Ying2,XIANG Yangfei2,QIAN Chuiwen2,REN Zhe2,WANG Yifei2,ZHANG Yingjun3,YANG Chongren3(1.College of Pharm acy,Jinan University,Guangzhou 510632,China,2.Biomedical Research , Development Center,3.State Key Laboratory of Phytochemistry , Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China) |
| Abstract: | Objective To investigate the in-vitro effect of the extract from Fructus toosendan on counteracting the activity of herpes simplex virus type 1(HSV-1). Methods By using Vero cell as the host cells and with acyclovir as the positive control,anti-HSV-1 activity of the extract was observed and determined with cytopathic effect (CPE) assay and plaque reduction assay. Initial studies on antiviral mechanism included three aspects:virucidal assay,attachment assay and penetration assay. Results The extract had cons... |
| Keywords: | Extract from Fructus toosendan MTT method CPE assay Plaque reduction assay |
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