| 基于DNA barcoding(条形码)技术的中药材鉴定 |
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| 引用本文： | 陈士林,姚辉,宋经元,李西文,刘昶,陆建伟. 基于DNA barcoding(条形码)技术的中药材鉴定[J]. 世界科学技术, 2007, 9(3): 7-12 |
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| 作者姓名： | 陈士林 姚辉 宋经元 李西文 刘昶 陆建伟 |
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| 作者单位： | [1]中国医学科学院 中国协和医科大学药用植物研究所,北京100094 [2]香港大学李嘉诚医学院分子中药实验室,香港 [3]国家中医药管理局科技司,北京100026 |
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| 摘 要： | DNA条形码（DNA barcoding）是根据对一段标准的DNA序列的分析来鉴定物种，已成为生物物种鉴定的新方向，受到世界40多个国家130多个组织中传统生物分类学家、分子生物学家和生物信息学家等多学科专家的关注。本文通过介绍DNA条形码的产生、发展和研究现状，探讨其在中药材鉴定中应用的技术方法、技术路线、关键问题以及应用范围，并展望了DNA条形码在中药材鉴定中的应用前景。
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| 关 键 词： | 中药材 DNA条形码 鉴定 |
| 收稿时间： | 2006-09-12 |
| 修稿时间： | 2006-09-122007-03-15 |
Use of DNA barcoding to identify Chinese medicinal materials |
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| Chen Shilin,Yao Hui,Song Jingyuan,Li Xiwen,Liu Chang,Lu Jianwei. Use of DNA barcoding to identify Chinese medicinal materials[J]. World Science and Technology(Modernization of Traditional Chinese Medicine), 2007, 9(3): 7-12 |
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| Authors: | Chen Shilin Yao Hui Song Jingyuan Li Xiwen Liu Chang Lu Jianwei |
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| Affiliation: | 1.Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100094, China;2.Molecular Chinese Medicine Laboratory, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pok Fu Lam, HongKong;3. State administration of traditional Chinese medicine of P. R. China, Beijing 100026 |
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| Abstract: | To identify biological species, DNA barcoding as a new technique is developed. DNA barcoding sequences from a standardized and agreed -upon position in the genome are very short relative to the entire genome and they can be obtained reasonably quickly and cheaply, which attracted more than 130 organizations from 40 countries in the globe. This paper reviews the appearance and development of DNA barcoding. Simultaneously, we study the methods, stepwise technique map and key questions of DNA barcoding which was used to identify Chinese medicinal materials. In conclusion, DNA barcoding has a good prospect in authentication of Chinese medicinal materials. |
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| Keywords: | Chinese medicinal materials DNA barcoding Identification |
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