| 应用高速逆流色谱技术从附子中分离制备苯甲酰新乌头原碱 |
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| 引用本文： | 吴平丽,刘雯,卓超,张继全,沈平孃.应用高速逆流色谱技术从附子中分离制备苯甲酰新乌头原碱[J].世界科学技术,2009,11(2):260-262. |
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| 作者姓名： | 吴平丽 刘雯 卓超 张继全 沈平孃 |
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| 作者单位： | 吴平丽,刘雯,卓超,Wu Pingli,Liu Wen,Zhuo Chao(华东理工大学药学院,上海,200237);张继全,沈平孃,Zhang Jiquan,Shen Pingniang(国家中药制药工程技术研究中心,上海,201203)
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| 基金项目： | 国家科技部科技支撑计划 |
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| 摘 要： | 利用高速逆流色谱技术，一次进柱即可实现从附子粗提物中分离得到苯甲酰新乌头原碱。两相溶剂系统用氯仿-甲醇-0.3mol／L盐酸三元系统，上相为固定相，下相为流动相，转速为892rpm，流速为1.2ml／min。通过ESI-MS、^13C-NMR,^1H-NMR鉴定，确认了其化学结构。经HPLC分析，纯度达98％以上。
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| 关 键 词： | 高速逆流色谱 附子 苯甲酰新乌头原碱 |
Separating Benzoylmesaconitine from Aconite Using HSCCC |
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| Wu Pingli,Liu Wen,Zhuo Chao,Zhang Jiquan,Shen Pingniang.Separating Benzoylmesaconitine from Aconite Using HSCCC[J].World Science and Technology(Modernization of Traditional Chinese Medicine),2009,11(2):260-262. |
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| Authors: | Wu Pingli Liu Wen Zhuo Chao Zhang Jiquan Shen Pingniang |
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| Institution: | Wu Pingli, Liu Wen, Zhuo Chao,Zhang Jiquan, Shen Pingniang(1.East China University of Science and Technology School of Pharmacy, Shanghai 200237;2.National Traditional Medicine Engineering Center, Shanghai 201203) |
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| Abstract: | separated Benzoylmesaconitine from raw aconite in an immediate manner using HSCCC. A methanol-methylene chloride-hydrochloric acid （-0.3mol/L） solvents system was used, with a rotation speed at 892rpm and a flow rate 1.2ml/min, making the upper part the fixed phase, and the lower part the mobile phase. The chemical composition was determined through ESI-MS, ^13C-NMR, and ^1H-NMR screening. HPLC test showed a purity as high as 98% or above. |
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| Keywords: | HSCCC aconite Benzoylmesaconitine |
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