三才封髓丹加减对阴虚火旺型口腔溃疡小鼠血清白细胞介素-1β、2、6的影响

目的观察三才封髓丹加减对阴虚火旺型口腔溃疡小鼠血清白细胞介素-1β(IL-1β)、白细胞介素-2(IL-2)、白细胞介素-6(IL-6)水平的影响,评价三才封髓丹加减对阴虚火旺型口腔溃疡小鼠的治疗效果,探究其作用机制,进一步为临床应用提供理论依据。方法将61只小鼠随机分为正常组(15只)和实验组(46只),给予实验组小鼠每日0.32 mg/g(体质量)甲状腺片混悬液,连续灌胃9 d建立阴虚火旺模型,第10 d用氢氧化钠(Na OH)晶体灼烧法,联合建立阴虚火旺型口腔溃疡小鼠模型。第11 d造模完成后,随机处死实验组小鼠1只,留取有损伤的口腔黏膜组织做病理切片,并将实验组小鼠45只随机分为模型对照组、维生素阳性对照组、三才封髓丹治疗组,各15只,按每日0.02 m L/g(体质量)分别灌胃给药,连续6 d。测定小鼠体质量、体温,检测血清中IL-1β、IL-2、IL-6的水平。结果实验组小鼠与正常组小鼠体质量变化比较差异有统计学意义(P0.05),正常组与实验组小鼠每日体温比较差异无统计学意义(P0.05)。口腔溃疡模型病理可见,表面被覆鳞状上皮,表层轻度角化,间质内纤维组织增生,小血管扩张,慢性炎细胞浸润,罕见涎腺组织。模型对照组小鼠血清IL-1β、IL-6水平较正常组明显升高(P0.05),IL-2水平较正常组明显下降(P0.05)。维生素对照组小鼠血清IL-1β、IL-6水平较模型对照组明显下降(P0.05),IL-2水平较模型组亦明显下降(P0.05)。三才封髓丹组小鼠血清IL-1β、IL-6水平较模型对照组明显降低(P0.05),其中IL-1β水平较正常组亦明显下降(P0.05),IL-2水平模型组明显升高(P0.05)。结论三才封髓丹加减可降低血清中IL-1β、IL-6含量,升高IL-2,对阴虚火旺型口腔溃疡具有一定的治疗作用,其作用机制可能与增强免疫作用有关。混合维生素可以降低血清中IL-1β、IL-6含量,对阴虚火旺型口腔溃疡有治疗作用,而过量的维生素会降低血清中IL-2,可能有抑制免疫的作用。

Effects of Sancai - Fengsui pills on serum IL - 1β,IL - 2 and IL - 6 in oral ulcer mice with fire excess from yin deficiency type

Objective To observe the effects of Sancai - Fengsui pills on serum interleukin(IL - 1β),IL- 2 and IL - 6 in mice model of oral ulcer with fire excess from yin deficiency type,to study its therapeutic effects and action mechanism,provide an further evidence for clinical application. Methods Mice were randomly divided into normal group and experimental group. Mice(n = 46)in experimental group received suspension of thyroxine 0. 32 mg/(g·d)]for 9 d which order to establish the model of fire excess from yin deficiency type,and then the o-ral ulcer model was established by NaOH crystal - burned at day 10. After successful modeling at day 11,termination of one mouse in experimental group took injury oral mucosa tissues for pathological section,the other mice in experi-mental group were randomly divided into model control group,vitamin positive control group and Sancai - Fengsui pills group,15 mice each group,continuously treatment intragastric administration0. 02 mg/(g·d)]for 6 d,re-spectively. The levels of body weight,temperature,serum IL - 1β,IL - 2 and IL - 6 were measured. Results There were statistical differences on body weight between experimental group which using thyroxine and normal group (P < 0. 05). There were no statistical differences on daily temperature between normal and experimental group( P >0. 05). The pathological section of oral ulcer model showed cover squamous epithelium,surface mild,interstitial fibrous proliferation,the minute vessel expands,chronic inflam-matory cells and rare salivary tissues. The levels of serum IL - 1β and IL - 6 in model control group were obviously higher than those in normal group(P < 0. 05),and the IL - 2 was obviously lower(P < 0. 05). The levels of serum IL - 1β and IL - 6 in vitamin positive control group were obviously lower than those in model control group(P < 0. 05),and the IL - 2 was obviously lower than that in model control group(P < 0. 05). The levels of serum IL - 1βand IL - 6 in Sancai - Fengsui pills group were obviously lower than those in model control group(P < 0. 05),and the serum IL - 1βwas obviously lower than that in normal group(P < 0. 05),the IL - 2 was obviously higher than that in model group(P < 0. 05). Conclusion Modified Sancai - fengsui pills can decrease the serum IL - 1β and IL - 6,increase IL - 2,has certain therapeutic effect on oral ulcer with fire excess from yin deficiency type. Its ac-tion mechanism may be related with immunological enhancement. Mixed vitamin can decrease serum IL - 1β and IL- 6,has therapeutic effect on oral ulcer with fire excess from yin deficiency type,but excess vitamin can decrease se-rum IL - 2,which may related with immune suppression.