姜黄素对急性肺栓塞大鼠的保护作用

目的观察姜黄素对急性肺栓塞大鼠的保护作用。方法将32只雄性清洁级SD大鼠随机分为4组,即假手术组、模型组、姜黄素低剂量组及姜黄素高剂量组,每组8只。模型组、姜黄素低剂量组、姜黄素高剂量组均采用颈总静脉注射自体血栓的方法制备肺栓塞模型,假手术组则注射0.9%氯化钠注射液。姜黄素低剂量组、姜黄素高剂量组腹腔注射姜黄素进行干预。取动脉血进行血气分析;测定肺系数、湿质量干质量(W/D);检测肺组织丙二醛(MDA)含量,超氧化物歧化酶(SOD)活性;放射免疫法检测血液中内皮素-1(ET-1)、血栓素B2(TXB2)、6-酮-前列腺素F1α(6-K-PGF1α)的含量。结果姜黄素高、低剂量组较模型组氧分压[p(O2)]、二氧化碳分压[p(CO2)]水平均升高(P0.01,P0.05),姜黄素低剂量组、姜黄素高剂量组肺系数、W/D、MDA含量、ET-1含量、TXB2/6-K-PGF1α(T/K)较模型组均降低(P0.01,P0.05),SOD活性较模型组升高(P0.01)。结论姜黄素对急性肺栓塞大鼠有保护作用。

Protection of Curcumine on acute pulmonary embolism of rat

Objective To investigate the protection of Curcumine on acute pulmonary embolism of rat.Methods 32 SD rats were randomly divided into four groups,sham group,model group,low dose Curcumine group and high dose Curcumine group.SD rats in model group,low dose Curcumine group and high dose Curcumine group were injected by carotid intravenous injection self-thrombus for establishment of pulmonary embolism model.Rat model in treatment groups were intervened by Curcumine.Arterial blood in rats was measured by blood gas analysis.Pulmonary coefficient,W/D,MDA,SOD,ET-1,TXB2 and 6-K-PGF1α were measured.Results PO2 and PCO2 in low dose Curcumine group and high dose Curcumine group were increased（P0.01 or P0.0.5）.Pulmonary coefficient,W/D,MDA,ET-1,TXB2 and 6-K-PGF1α were decreased in comparison with those in model group（P0.01 or P0.05）.ET-1 was increased（P0.01）.Conclusion Curcumine has protection on acute pulmonary embolism of rat.