Topical application of porcine placenta extract inhibits the progression of experimental contact hypersensitivity |
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Authors: | Jash Arijita Kwon Ho-Keun Sahoo Anupama Lee Choong-Gu So Jae-Seon Kim Jungho Oh Yu-Kyoung Kim Young Bong Im Sin-Hyeog |
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Institution: | a School of Life Sciences and Immune Synapse Research Center, Gwangju Institute of Science and Technology (GIST), 1 Oryong-dong, Puk-ku, Gwangju 500-712, Republic of Korea b Department of Life Science, Sogang University, Seoul 121-742, Republic of Korea c College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea d Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University, Seoul 143-701, Republic of Korea |
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Abstract: | Aim of studyPlacenta extract features as a composition of ointments used for skin beautification, dermatological diseases and skin dryness. However, little evidence has been cited about its underlying mechanisms of action by which it exerts a beneficial role in dermatological diseases in vivo. In this study, we intended to test the effect of topical application of porcine placenta extract in mouse model of contact hypersensitivity and elucidate its mechanism of action.Materials and methodsTo test the in vitro effect of porcine placenta extract, RAW 264.7 cells were cocultured with porcine placenta extract and stimulated with LPS (1 μg/ml) and the expression of inflammatory mediator TNF-α was estimated by RT-PCR at the mRNA level and by intracellular staining at the protein level. To further test in vivo efficacy, porcine placenta extract was topically applied to the mice with experimental skin hypersensitivity. For in vivo studies placenta extract in gel form was topically applied to ear of DNCB-induced contact hypersensitivity mouse model everyday for 2 weeks and progression of the disease was estimated by following criteria: (a) ear thickness, (b) serum IgE level by ELISA, (c) histological examination of ear tissue by H&E staining and (d) cytokine profile of total cells and CD4+ T cells by real time PCR.ResultsTopical application of porcine placenta extract on mouse ears with contact hypersensitivity decreased the severity and progression of the disease manifested by reducing ear swelling, inflammation and edema. Histological evaluation showed that placenta extract treatment reduced lymphocyte infiltration in the ear tissues. Protective effect of placenta extract is also associated with down-regulation of serum IgE level and inflammatory cytokine production (IL-1β, IFN-γ, TNF-α, IL-4, IL-12 and IL-17) in total lymph node cells and CD4+ T cells.ConclusionsOur data indicate that protective effect of porcine placenta extract in contact hypersensitivity is mediated by inhibition of the inflammatory responses and IgE production, suggesting a potential therapeutic application of porcine placenta extract to modulate skin inflammation. |
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Keywords: | PE placenta extract LPS lipopolysachharide TNF-α tumor necrosis factor alpha IL-1β interleukin 1 beta IFN-γ interferon gamma IL-4 interleukin 4 IL-6 interleukin 6 IL-2 interleukin 2 IL-17 interleukin 17 CHS contact hypersensitivity LC Langerhan's cell APC antigen presenting cell DLN draining lymph node NK cells natural killer cells DNCB 2 4-dinitrocholorobenzene OX oxazolone PBS phosphate buffer saline LPS lipopolysachharide PMA phorbol 12-myristate 13-acetate |
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