Canna indica L. attenuates high-glucose- and lipopolysaccharide-induced inflammatory mediators in monocyte/macrophage |
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Authors: | Heng Jung Chen Cheng-Nan Chen Mao-Lin Sung Yi-Chien Wu Pei-Ling Ko Tim K Tso |
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Institution: | 1. Department of Food Science, National Chiayi University, 300 University Road, Chia-Yi 60004, Taiwan, R.O.C;2. Nursing Department, Chung Jen College of Nursing, Health Science and Management, Chia-Yi, Taiwan, R.O.C;3. Department of Biochemical Science and Technology, National Chiayi University, Chia-Yi, Taiwan, R.O.C;4. Department of Cardiology, St. Martin de Porres Hospital, Chia-Yi, Taiwan, R.O.C |
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Abstract: | Ethnopharmacological relevanceCanna indica L. (CI) has been widely used as a folklore medicine in tropical and subtropical areas with beneficial effects in numerous diseases, including infection, rheumatism, hepatitis, and it has also been identified as an antioxidant.Materials and methodsThe present study aimed to investigate the effect of Canna indica CI ethanolic extract (CIE) on productions of nitric oxide (NO), prostaglandin E2 (PGE2), and interleukin-1β (IL-1β) in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. In addition, the effects of CIE in high glucose (HG)-induced U937 monocytes on mRNA expressions of IL-8 and monocyte chemoattractant protein-1 (MCP-1), and regulation of mitogen-activated protein kinase (MAPK) pathways were also identified.ResultsCIE was found to inhibit the production of inflammatory mediators including NO, IL-1β, and PGE2 from LPS-induced RAW 264.7 macrophages. The increases in HG-induced mRNA expressions of IL-8 and MCP-1 were also significantly inhibited by CIE. Stimulation of HG in U937 monocytes resulted in activation of p38 MAPK, ERK1/2, and JNK. However, CIE treatment significantly decreased phosphorylation of p38 MAPK, ERK1/2, and JNK.ConclusionThe present study demonstrated that CIE suppressed the LPS-induced inflammatory mediator production and also inhibited HG-induced inflammatory mediator expression by the regulation of MAPK pathway. |
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Keywords: | Canna indica Interleukin-8 Monocyte chemoattractant protein-1 Mitogen-activated protein kinase Prostaglandin E2 |
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