大黄素对肺纤维化大鼠TGF-β_1及smad_（3/7）信号通路的影响

目的：观察大黄素抗博莱霉素诱导大鼠肺纤维化的作用机制。方法：选用健康Wistar大鼠60只,随机分为空白组、模型组、强的松组、大黄素高、中、低剂量组,采用博莱霉素气管内吹入法,制备肺纤维化模型,于实验第29天留取标本,用免疫组化技术检测转化生长因子β1（TGF-β1）及smad3、smad7的表达。结果：大黄素中、低剂量组大鼠TGF-β1、smad3表达减弱,smad7表达明显增强。结论：大黄素的中、低剂量组能够减轻博莱霉素导致的大鼠肺纤维化程度,其作用机制与调控TGF-β1的smad3/7信号转导蛋白表达有关。

The Impact of Emodin on Transforming Growth Factorβ1 （TGF- β1）and smad3/7 Signaling Pathway of Pulmonary Fibrosis in Rats

Objective： To study the mechanism of emodin which treat pulmonary fibrosis induced by bleomycin in rats. Metheods： The healthy Wistar rats （60） were randomly divided into normal group, model group, prednisone group and emodin group （small dose, moderate dose and large dose）. Bleomycin was injected in bronchia to make the model of pulmonary fibrosis in rats. After 28 days,the rats were killed and collecte specimens to measure the expression of TGF-β1 and smad3 smad7. Results ： In emodin groups of small and moderate dose, The expression of TGF -β1 and smad3 is weak- ened ; and the expression of smad7 has enhanced. Conclusion ： Emodin has a certain treatment effect on the pulmonary fibrosis induced by bleomycin in rats, possibly through controlling TGF - β1 smad3/7 expression.