留针时间对肾阳虚家兔皮质醇及生命质量的影响

目的:探索留针时间长短与肾阳虚证的疗效关系。方法:将31只家兔随机分成A.空白对照组;B.模型空白对照组;C.留针时间15min组;D.留针时间30min组;E.留针时间40min组。分组后,除A组外,各组动物于每天上午在腹部皮下注射氢化可的松25mg/kg,连续注射7天。从第11天开始,A、B组只给予正常饲养,C、D、E除给予正常饲养外,按不同的留针时间要求分别给予针刺治疗观察,连续针刺10次。各组家兔分别在造模前(注射氢化可的松前)、造模后(注射氢化可的松后)及治疗10次后3次采血,每次均取清晨空腹血1ml用于检测皮质醇。结果:(1)留针时间15min组皮质醇显著高于留针时间40min组(P<0.05);留针时间15min组与留针时间30min组对比有明显差异(P<0.05);而留针30min组与留针40min组对比无明显差异(P>0.05)。(2)各组家兔生命质量积分比较,空白对照组明显低于模型组与针刺组(15,30及40min)(P<0.05),模型组与针刺组比较,(P<0.05);而留针15min与30min组及40min组间相比无明显差异(P>0.05)。结论:适当地缩短留针时间可以提高针刺治疗肾阳虚证患者的疗效。

Influence of Retaining Needle Time on the Kidney-yang Debility and Life Quality of Domestic Rabbit's Cortisol

Objective: To study the effects of retaining needle time on the Kidney-yang debility patients. Methods: 31 domestic rabbits were randomly divided into five groups: A, a blank control group; B, a blank model control group; C, 15-minute retaining needle group; D, 30-minute retaining needle group; E, 40-minute retaining needle group. Each group was given an interjection of 25 mg/kg of hydrocortisone in the belly in the morning for seven days except Group A. From the 11th day on, Group A and B were fed normally while the Group C, D and E were given acupuncture treatment for ten times according their retaining needle time besides normal feeding. Each group was collected blood for three times before and after the mold built (interjection of hydrocortisone) as well as after ten times treatment respectively. Each time 1 ml of fasting blood was taken to test the cortisol. Results: 1. The cortisol of 15-minute retaining needle group is higher than that of 40-minute group (P <0.05). The contrast between the 15-minute and 30-minute groups has a significance difference (P <0.05) while the contrast between the 30-minute and 40-minute groups has no obvious difference (P <0.05) 2. By the calculus comparison of the life quality of each group, the blank control group is obviously lower than the model group and the acupuncture group(P <0.05). The comparison between the model group and the acupuncture group(P <0.05) while the comparison among the retaining needle groups (15 , 30, 40 min) has no obvious difference (P <0.05). Conclusion: The proper reduction of the retaining needle time may improve the curative effectiveness of the acupuncture treatment of Kidney-yang debility patients.