“双固一通”针法对绝经后骨质疏松症模型大鼠生化指标的影响

目的:研究“双固一通”针法对绝经后骨质疏松症模型大鼠骨代谢生化指标的影响.方法:按随机原则将50只SD大鼠分为对照组、模型组、常规组、双固一通组和中成药组,每组各10只,模型组和常规组、双固一通组、中成药组切除双侧卵巢后造成实验性绝经后骨质疏松症动物模型.测定血清Ca、P、ALP和尿Ca、P、Cr;测定血BGP;测定尿DPD的含量.结果:模型组血Ca值明显降低、血P值明显升高、BGP明显升高(P＜0.01);血ALP值升高(但未达显著性差异).与模型组比较,双固一通组和中成药组血Ca明显升高、血P明显降低、BGP明显升高(P＜0.01),双固一通组优于常规组(P＜0.01);血ALP降低(但未达显著性差异).模型组尿Ca/Cr、P/Cr比值明显升高(P＜0.01);与模型组比较,各组Ca/Cr、P/Cr比值均降低(P＜0.01).模型组尿DPD明显升高(P＜0.01).与模型组比较,双固一通组尿DPD含量明显降低(P＜0.01).结论:“双固一通”针灸治疗后,BGP显著升高,提示成骨细胞活性增强,骨形成增加;血钙升高,血磷降低,尿Ca/Cr、尿P/Cr、DPD降低,提示“双固一通”针灸治疗可以通过增加胃肠吸收功能,促进对钙、磷等各种营养物质的吸收和利用,调节Ca、P代谢,降低尿I型胶原分解产物DPD浓度,提高BGP浓度,从而抑制骨吸收、促进骨形成.说明中医药治疗可以调节Ca、P代谢,促进骨形成,减缓骨吸收.

Effect of Double-reinforcing and One-unblocking Techniques of Needling on Biochemical Indicators in Rat Model of Postmenopausal Osteoporosis

Objective： To investigate the effect of Double-reinforcing and One-unblocking techniques of needling on biochemical indicators of bone metabolism in rat model of postmenopausal osteoporosis. Methods： 50 SD rats were divided into five groups randomly, which were control group, model group, normal group, treatment group and Chinese patent medicine group of 10 rats each group. In model group, normal group, treatment group and Chinese patent medicine group the rats were made into the model of experimental postmenopausal osteoporosis by ovarieetomy. Then Ca, P and ALP in serum, Ca, P and Cr in urine, BGP in blood and DPD in urine were de- termined. Results： In model group Ca decreased and P increased markedly, and BGP increased remarkably too（P 〈0.01 ）. ALP increased but not markedly. Compared with in model group, Ca increased, P decreased and BGP increased all remarkably in treatment group and Chinese patent medicine group（ P 〈 0.01 ）, but treatment group did better than normal group（P 〈0.01 ）. ALP decreased but not markedly. In model group Ca./Cr and P/Cr increased markedly（P 〈0.01 ）. Compared with model group Ca/Cr and P/Cr in each group decreased（P 〈0.01 ）. In model group DPD increased significantly（P 〈0.01 ）. But compare with model group DPD in urine in treatment group decreased significantly（ P 〈 0.01 ）. Conclusion： BGP was increased significantly after treatment with Doublereinforcing and One-unblocking techniques of needling, which shows that osteoblast activity is strengthened and bone formation increases. Ca increases, P decreases, Ca/Cr, P/Cr and DPD decrease which suggest that Doublereinforcing and One-unblocking techniques of needling function, stance to can promote encourage Ca, gastrointestinal absorptive P and other nutrient subbe absorbed and utilized, regulate the metabolism of Ca and P, cut down DPD concentration which is urinary type I collagen decomposition products, and increase BGP concentration so as to inhibit bone resorption and promote bone formation. This means that traditional Chinese medicine treatment can regulate the metabolism of Ca and P, promote bone formation, and slow down the bone absorption.