肾络康煎剂对实验性IgA肾病模型大鼠TNF-α蛋白表达的影响

目的：探讨肾络康煎剂治疗IgA肾病（IgAN）的作用机理。方法：采用脂多糖（LPS）＋牛血清白蛋白（BSA）＋四氯化碳（CCl4）作为造模方法制作IgA肾病大鼠模型,并分别给予肾络康煎剂和贝纳普利治疗,最后用免疫组化的方法观察各组大鼠肾组织TNF-α蛋白表达。结果：模型组大鼠肾小球IgA荧光较正常,显著组增强（u=3.464 1,P〈0.01）,而肾络康煎剂组较模型组明显减弱（u=2.327 9,P〈0.05）;简络康组肾小球系膜细胞数43.75±9.68与模型且62.53±15.33比较（t=-2.929 8,P〈0.05）,差异显著;肾络康组TNF-α阳性面积（%）为31.25±11.67,较模型组60.67±13.27显著降低（t=-4.708 8,P〈0.01）。结论：肾络康煎剂抑制IgA肾病模型肾组织TNF-α蛋白表达可能是其治疗IgA肾病的作用机制之一。

The influence of Decoction of Shen-luo-kang on TNF-α Albumen Expression in Experimental Models of IgA Nephropathy

Objective： To investigate the mechanism of action of Decoction of Shen - luo - kang on IgA nephropathy. Method： The models of IgA nephropathy were made with lipopolysaccharide, bovine serum albumin and carbon tetrachloride （ CCl4）. Respectively Decoction of Shen - luo - kang and benazepril were given to the models. At last TNF - α albumen expression in nephridial tissue in each group was observed with immunohistochemical method. Resuh：IgA fluorescence in rat＇s glomcrulus in model group was strengthened than that in normal group（u =3.464 1 ,P 〈 0. 01 ）, but was weakened obviously in treatment group than that in model group（u = 2. 327 9, P 〈 0. 05 ）. Mesangial cell number in treatment group was （43.75 ± 9.68 ）, and （62. 53 ± 15.33 ）in model group. The difference was significant（ t = -2. 929 8, P 〈 0. 05 ）. TNF-α positive area in nephridial tissue inhibited by Decoction of shen - luo - kang was （ 31.25 ± 11.67 ） , lower than that in control group which was （ 60. 67 ± 13.2） , （t = - 4. 708 8,P 〈 0. 01 ）. Conclusion ： Decoction of Shen - luo - kang can inhibit TNF - α albumen expression in nephridial tissue in the models of IgA nephropathy thus to decrease urine protein. It is one of effective treatment methods.