三七对胰岛素抵抗大鼠脂肪细胞因子的影响

观察三七对胰岛素抵抗（IR）大鼠血清脂肪细胞因子的影响，探讨三七抗IR的作用机制。方法：采用高脂膳食复制IR大鼠模型，全自动生化分析仪测定大鼠空腹血糖（FBG）水平，放射免疫法检测血清空腹胰岛素（FINS）、IL-6水平，ELISA法检测血清TNF—α、kptin水平，酮试荆法检测血清FFA水平。结果：模型组大鼠血清FINS较正常组明显增高，而胰岛素敏感性ISI明显低于正常组；血清TNF-仪、FFA水平较正常组明显增高，FBG、Leptin、IL-6水平与正常组差异无显著性；应用三七干预后，大鼠ISI较模型组明显提高，FINS、TNF—α、FFA水平较正常组明显降低。结论：调节脂肪细胞因子TNF—α、FFA的分泌可能是三七改善大鼠IR的作用机制之-。

EFFECTS OF PANAX NOTOGENSENG ON ADIPOCYTOKINES IN INSULIN RESISTANCE RATS

Objective:To observe the effects of panax notogenseng on serum adipocytokines in insulin resistance rats and discuss the mechanism of panax notogenseng on insulin resistnnce. Methods:The insulin resistance model was induced by high fat diet, and the fasting blood-glucose (FBG) was tested by automatic biochemistry analyzer, the fasting insulin(FINS)and IL-6 were tested by radioimmunoassay, TNF-alpha and Leptin contents were determined by ELISA, FFA was tested by ketone reagent method. Results:Serum FINS of model group was significantly higher than that of normal, insulin sensitivity index (ISI) was obviously lower than that of normal; the levels of serum TNF-alpha and FFA were significantly higher than those in normal group ,however the levels of FBG,,Leptin and IL-6 showed no significant difference between the normal and the model groups. ISI was significantly raised and FINS, TNF-α,FFA were reduced after the the treatment of Panax notogenseng. Conclusion:Panax notogenseng can improve the secretion of adipocytokines, which may be one of the mechanisms that panax notogenseng improve rats’ insulin resistance