青蒿素对实验性糖尿病大鼠肾脏蛋白激酶C移位活化的抑制作用

目的探讨青蒿素对实验性糖尿病大鼠肾脏蛋白激酶C（PKC）由细胞浆到细胞膜移位活化的抑制作用。方法将实验大鼠随机分为对照组（A组）、糖尿病非治疗组（B组）及糖尿病青蒿素治疗组（C组），B组、C组采用腹腔单剂量注射链脲佐菌素（STZ）65mg／kg的方法建立糖尿病大鼠模型，造模后c组采用青蒿素300mg／（kg·d）腹腔注射。于实验进行到第3周、第6周时分别宰杀大鼠6只，测算肌酐清除率（Ccr）、24h尿白蛋白排泄率（UAER）及肾脏系数（肾脏质量／体质量）；采用酶联免疫吸附法（ELISA）测定PKC的活性。结果B组大鼠Ccr、UAER、肾脏细胞膜PKC活性及肾脏系数均较A组明显升高（P〈0．05或0．01）；C组大鼠上述指标均较B组明显下降（P〈0．05或0．01）。结论青蒿素通过抑制糖尿病大鼠肾脏细胞PKC移位活化而对糖尿病大鼠早期肾脏病变起到保护作用。

Inhibitory effect of artemisinin on the spatiotemporal dynamics activation of protein kinase C in the kidney of experimental diabetic rats

Objective It is to approach the inhibitory effect of artemisinin on the spatiotemporal dynamics activation of protein kinase C （PKC） in the kidney of experimental STZ - induced diabetic rats. Methods The experimental rats were divided into control group （group A） , diabetic without treatment group （group B） and diabetic with treatment of artemisinin group （group C）. The model of diabetic rats was established by intraperitoneal injection of a single close of 65 mg/kg induced by STZ. After modeling, the animals in group C were treated with artemisinin of 300 mg/（ kg·d） by intraperitoneal injection. UAER, Ccr and Kidney coefficient were observed after 3 - and 6 - week of treatment, while the endogenous PKC activity was measured by ELISA. Results Kidney coefficient, Ccr, UAER and PKC activity at the plasma membrane in group B were significantly higher than those in group A （P 〈 0.05 or 0.01 ）; meanwhile, these indexes in group C were markedly lower than those in group B （ P 〈 0.05 or 0.01 ）. Conclusion Artemisinin has some renoprotective effects in experimental STZ - induced diabetic rats through inhibiting the spatiotemporal dynamics activation of PKC in the kidney.