“以方测证”验证化浊解毒对溃疡性结肠炎大鼠血清TNF-α、IL-1β的影响

目的观察解毒消痈方药对溃疡性结肠炎大鼠血清TNF—α、IL-1β的影响。方法将SD大鼠随机分为正常组、模型组、解毒消痈方药大剂量组、解毒消痈方药中剂量组、解毒消痈方药小剂量组、阳性对照柳氯磺胺吡啶组，每组各10只。以三硝基苯磺酸建立溃疡性结肠炎大鼠动物模型，采用双抗体夹心法检测大鼠血清TNF—α、IL-1β的变化。结果大鼠血清肿瘤坏死因子“及白介素1β的含量模型组均明显高于正常组（P均〈0．05）；解毒消痈方药大、中剂量组明显低于模型组（P均〈0．05）。结论解毒消痈方药有下调促炎因子TNF-α及IL-1β的作用，存在效量关系，剂量增加能提高疗效，其抗炎机制可能与下调TNF—α及IL-1β含量有关。

The verification in influence of Huazhuojiedu on serum TNF-α, 1L-1β ：in rats with ulcerative colitis by ＂Syndrome Differentiation through Formula Effect Assessment＂

Objective It is to observe the influence of the detoxification eliminated carbuncle recipe （DECR） on serum tumor necrosis factor - α （TNF - α）, interleukin - β （ IL - 1β） in rats with ulcerative colitis. Methods 60 SD rats were random- ly divided into normal group, model group, DECR high-dose group, DECR medium-dose group, DECR low-dose group, positive control sulfasalazine group. There were 10 rats in each group. Rat model of ulcerative colitis was established by trini- trobenzene sulfonic acid, and the changes of serum TNF- α, IL - 1β by double-antibody sandwich assay. Results The levels of serum TNF- α and IL- 1β in model group were significantly higher than that in normal group （P〈0.05）. The levels in DECR high-dose and medium-dose groups were significantly lower than that in model group（P 〈 0.05 ）. Conclusion DECR have reduced detoxification pro-inflammatory cytokines TNF-α and IL- 1β in the role, there is an efficiency-amount relationship, the dose increases can improve the effects, its anti-inflammatory mechanism may be related to reduced the levels of TNF- α and IL- 1β.