中医痰证与载脂蛋白A5基因-1131T〉C多态性的关系研究

目的探讨载脂蛋白A5（ApoA5）-1131T〉C基因多态性与中医痰证的关系。方法运用聚合酶链反应-限制性片段长度多态性分析技术对湖南地区131例痰证患者及74例健康人的ApoA5-1131T〉C基因型进行检测,同时检测所有研究对象的血脂、血压及体质量指数。结果 205例受检者中,ApoA5-1131T〉C位点TT型76例（37.07%）,TC型94例（45.85%）,CC型35例（17.07%）,T等位基因频率60.00%,C等位基因频率40.00%。CC型血清三酰甘油水平明显高于TT型（P〈0.01）。病例-对照研究及多因素Logistic回归分析均支持ApoA5-1131C是痰证发生的独立危险因素。结论 ApoA5-1131C可能是痰证发生的独立危险因素。ApoA5-1131CC型可能与血清三酰甘油水平升高有关。

Study on the relationship between apolipoprotein A5 gene - 1131T 〉 C polymorphism and phlegm stagnation syndrome

Objective It is to explore the relationship between the polymorphisms of apolipoprotein A5 （APOA5） gene （ -1131T 〉 C ） and phlegm stagnation syndrome（PSS）. Methods Patients of PSS（ n = 131 ） and healthy controls（ n = 74） were grouped and compared in this study. Using polymerase chain reaction-restriction fragment length polymorphisms （PCR - RFLP） technique to analyze the APOAS（ -I131T 〉 C） genotypes. Serum lipids, blood pressure（BP） and body mass index（BMI） in the pa- tients and controls were also measured. Results The frequencies of TT, TC, CC genotypes for the APOA5 - 1131T 〉 C poly- morphism were 37.07% （ n = 76） , 45.85% （ n = 94） and 17.07% （ n = 35）. The frequency of T allele was 60.00% and the frequency of C allele was 40.00%. The serum triglyceride （TG）level of - 1131 CC genotype carries were significantly higher than -I131TT genotype carries （P 〈 0.01 ）. Patients-controls research and muhinomial-logistic analysis indicated that- 1131 C was a risk factor of the PSS. Conclusion The - 1131 C allele of APOA5 gene is possibly the independent risk factor of the SPS. The - 1131CC genotype is possibly associated with the higher level of serum triglyceride.