通腑化瘀汤对术后肠梗阻大鼠小肠动力及IL-6、IL-10的影响

目的]研究通腑化瘀汤对术后肠梗阻(POI)模型大鼠小肠动力及血清白介素(IL)-6、IL-10的影响,探讨中药对促进POI胃肠功能恢复的作用。方法]实验动物按体质量随机分为空白组、对照组和药物组,手术建立大鼠POI模型。药物组给予通腑化瘀汤灌胃,对照组给予等量的生理盐水,空白组不予干预。术后24 h测定各指标。结果]与空白组相比,对照组的小肠推进率显著降低,血清IL-6水平显著升高,血清IL-10也升高(P0.05);与对照组相比,药物组的小肠推进率显著升高,血清IL-6水平显著降低,血清IL-10显著升高(P0.05);且小肠推进率与血清IL-6浓度呈负相关,与血清IL-10浓度呈正相关。药物组肠黏膜的病理评分也低于对照组(P0.05)。结论]通腑化瘀汤可改善小肠动力,降低促炎因子IL-6同时提高抗炎因子IL-10的表达,从而缓解POI,保护胃肠功能。

Effects of Tongfu Huayu decoction on small intestine motility, IL-6, and IL-10 in postoperative ileus rats

Objective] To research the influence of decoction on small intestine motility, IL-6 and IL-10 in postoperative(POI) rat, discussing the promoting effect of traditional Chinese medicine for gastrointestinal functional recovery. Methods] Forty-five rats were randomly divided into normal group, NS group and Tongfu Huayu group. The animal model of postoperative ileus was established by laparotomy in rats. Rats of NS group and Tongfu Huayu group were perfused saline and equivalent Tongfuhuayu potions separately. The normal group would not be operated. Twenty-four hours after surgery, the rats were sacrificed, then small bowel propulsion rate, serum IL-6 and IL-10 level were measured, and got a length of small bowel tissue nearby ileocecus making hematoxylin-eosin staining. Results] The small bowel propulsion rate were reduced significantly in NS group, the serum IL-6 level increased significantly in NS group, the serum IL-10 level increased significantly in NS group, comparing to the normal group(P<0.05). And the small bowel propulsion rate were improved significantly in Tongfu Huayu group. The serum IL-6 level were improved significantly in Tongfu Huayu group. The serum IL-10 level were more increased significantly in Tongfu Huayu group, comparing to the NS group(P<0.05). The small bowel propulsion rates were negatively correlated with the serum IL-6, and were positively correlated with the serum IL-10. The intestinal mucosa of rats was normal in normal group, and it was more injured in NS group than in Tongfu Huayu group(P<0.05). Conclusion] Administration of Tongfu Huayu decoction after surgery may prevent surgery-induced ileus through the effect on the decrease of serum IL-6 and the increase of serum IL-10. Also,Tongfu Huayu decoction can relieve the injury of intestinal mucosa caused by surgery and develop impact of protection.