七子益肾理冲汤对DOR大鼠卵巢储备的影响

目的]探讨七子益肾理冲汤对脱氧乙烯基环己烯(VCD)致卵巢储备功能低下(DOR)大鼠卵巢储备功能的影响。方法]60只雌性SD大鼠随机分为空白组、模型组、中药低、中、高剂量组及脱氢表雄酮(DHEA)组。模型组采用VCD注射制作DOR大鼠模型。观察卵巢指数,HE染色观察卵巢组织形态,放射免疫法检测促卵泡刺激素(FSH)、血清雌二醇(E2)及抗穆勒管激素(AMH)水平。结果]与空白组比较,模型组卵巢组织萎缩,各级卵泡数目减少,卵巢指数和E2、AMH水平降低,FSH水平升高(P0.05);与模型组比较,中药高剂量组卵巢指数上升,卵巢组织形态明显改善,原始卵泡和黄体增加,闭锁卵泡减少;E2、AMH水平升高,FSH水平降低(P0.05)。结论]七子益肾理冲汤可修复卵巢储备的损失,促进卵泡生长,抑制卵泡闭锁。

Effect of Qizi Yishen Lichong decoction on ovarian reserve in rats model

Objective] To investigate the effect of Qizi Yishen Lichong decoction on ovarian reserve in DOR rats model.Methods] The 60 female SD rats were divided into six groups:blank group, model group, CM low-dose group, CM normal-dose group, CM high-dose group and DHEA group. The ovarian index was calculated, the ovarian HE slides were observed under microscope, and blood FSH, E₂ and AMH levels were tested by Elisa.Results] Compared with blank group, model group had shown ovarian tissue atrophy in HE slides, numbers of all types of ovarian follicles declined, FSH level increased significantly, while ovarian index, E₂ and AMH levels significantly lowered (P<0.05); compared with model group, CM high-dose group has shown a better ovarian appearance in HE slides, increased in numbers of ovarian primordial follicles and corpus luteum, decreased in numbers of atresia follicles, ovarian index, E₂ and AMH levels significantly increased (P<0.05), and significantly drop of FSH level (P<0.05).Conclusion] Qizi Yishen Lichong decoction might repair the loss of ovarian reserve, stimulate the growth of ovarian follicles and reduce ovarian follicle apoptosis.