慢性再生障碍性贫血辨证分型与Fas/FasL蛋白表达相关性研究

目的]探讨慢性再生障碍性贫血中医辨证分型与Faa、FasL蛋白表达的相关性,解析慢性再生障碍性贫血辨证分型的物质基础.方法]选择90例慢性再生障碍性贫血患者,其中脾肾阳虚、肾阴阳两虚、肝肾阴虚者各30例;另设15例健康志愿者为正常对照组.应用免疫印迹杂交技术检测慢性再生障碍性贫血患者和正常人骨髓Fas、FasL蛋白表达.结果]慢性再生障碍性贫血患者Fas蛋白、FaaL蛋白的表达高于正常对照组(P＜0.05或P＜0.01),而且脾肾阳虚组、肾阴阳两虚组与肝肾阴虚组组间Fas蛋白、FaaL蛋白的表达存在明显差异P＜0.05或P＜0.01),按脾肾阳虚组、肾阴阳两虚组与肝肾阴虚组次序依次升高.结论]慢性再生障碍性贫血的辨证分型与Fas、FasL蛋白表达存在相关性,可作为慢性再生障碍性贫血辨证分型的物质基础.

Correlation between differentiated classification of chronic aplastic anaemia and Fas/FasL protein expression

Objective] To study the correlation between differentiated classification of chronic aplastic anaemia and Fas/FasL protein expression, to analyze the material basis of differentiation of cases with chronic aplastic anemia by TCM. Methods] Ninety patients of chronic aplastic anemia, including 30 cases of asdthenic splenonephro-yang, 30 cases of kidney asthenia of both yin and yang, 30 cases of hepatic and renal yin deficiency, were selected in this study. The Fas/FasL protein expression in the bone marrow of CAA was detected by western blot. Results] Fas/FasL protein expression in bone marrow of chronic aplastic anemia patients was higher than control (P<0.05, P<0.01). Among these three syndromes, Fas/FasL expression of hepatic and renal yin deficiency was the highest, patients with deficiency of both yin and yang was higher, asdthenic splenonephro-yang was high, all had significant difference (P<0.05,P<0.01). Conclusion] The differentiation of cases by traditional Chinese medicine and Fas/FasL protein expression of chronic aplastic anaemia was correlative, Fas/FasL protein can be represented as the substantial basis of the syndrome differentiation of CAA.