首页 | 本学科首页   官方微博 | 高级检索  
     

金莲花蛋白超声提取工艺优化及其抑菌活性的初步测定
引用本文:张丽娟,张贵君,李仁伟. 金莲花蛋白超声提取工艺优化及其抑菌活性的初步测定[J]. 天津中医药, 2007, 24(1): 63-65
作者姓名:张丽娟  张贵君  李仁伟
作者单位:1. 首都医科大学燕京医学院生化教研室,北京,101300
2. 北京中医药大学,北京,100102
基金项目:教育部科学技术研究项目
摘    要:[目的]优化金莲花(Flos Trollii Chinensis)蛋白超声提取工艺,并测定蛋白提取液的抑菌活性。[方法]用L9(34)正交设计优化超声提取金莲花蛋白工艺,用纸片扩散法检测金莲花蛋白抑菌活性。[结果]金莲花蛋白超声提取的最佳工艺为:原料粒度60目,料液比1∶30,提取时间30min。金莲花蛋白提取液(1.25g/L)具有抑制变形杆菌( )、伤寒杆菌( )、产气杆菌( )和大肠杆菌( )的作用。[结论]利用超声提取方法能够获得高产率的金莲花蛋白,并能保持其生物活性。

关 键 词:金莲花蛋白  抑菌活性  提取工艺
文章编号:1672-1519(2007)01-0063-03
收稿时间:2006-10-20
修稿时间:2006-10-20

Optimization of process for ultrasonic extraction and primary determination of bacteriostatic activity of protein from flos trollii chinensis
ZHANG Li-juan,ZHANG Gui-jun and LI Ren-wei. Optimization of process for ultrasonic extraction and primary determination of bacteriostatic activity of protein from flos trollii chinensis[J]. Tianjin Journal of Traditional Chin Medicine, 2007, 24(1): 63-65
Authors:ZHANG Li-juan  ZHANG Gui-jun  LI Ren-wei
Affiliation:1.Teaching and Research Section of Biochemistry, Yanjing Medical Institute of Capital Medical University, Beijing 101300, China; 2.Beijing University of TCM, Beijing 100102, China
Abstract:[Objective] To optimize the ultrasonic extraction technology for the protein from Flos Trollii Chinensis and determinate the bacteriostatic activity of the protein extract.[Methods] By applThe L9(34) orthogonal test was applied to optimize the ultrasonic extraction technology and the activity of bacteriostasis was determined with slip diffusion method.[Results] The optimized ultrasonic extraction technology was as follows: material granule 60 order,proportion of material to solution 1:30,extracted time 30 min.The extracted protein at 1.25 g/L could inhibit Bacillus proteus(++),Bacillus typhosus(++),Bacillus aerogenes(+),Bacterium coli(+).[Conclusions] The method with ultrasonic extraction can obtain higher plurinatality of protein of Flos Trollii Chinensis without any loss of its biologic activity.
Keywords:protein from Flos Trollii Chinensis  restrain bacterium action  extraction technical
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《天津中医药》浏览原始摘要信息
点击此处可从《天津中医药》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号