清脂通脉颗粒改善家兔血脂及抗动脉粥样硬化随机平行对照研究

目的]探讨清脂通脉颗粒对家兔血脂、主动脉粥样硬化影响及机制。方法]建立高脂家兔模型。将48只家兔按随机数字表法分为6组：空白对照组、模型组、血脂康组、清脂通脉颗粒低剂量组、清脂通脉颗粒中剂量组、清脂通脉颗粒高剂量组，8只／组。造模成功后，除空白对照组外，其它各组灌胃干预，15mL／kg·次，2次／d。模型组予生理盐水；血脂康组予血脂康0．1g／kg·d；清脂通脉颗粒各剂量组，分别为0．69g／kg·d（3g生药／kg·d），2．31g／kg·d（10g生药／kg·d）以及6．94g／kg·d（30g生药／kg·d）。连续灌胃干预10周。观测各组大鼠血清甘油三脂（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）。结果]模型组4项血清值检测结果均高于空白对照组（P〈0．05）。清脂通脉颗粒中、高剂量组甘油三酯（TG）均明显低于模型组（P〈0．05），与实验用剂量的血脂康作用相近（P〉0．05）。清脂通脉颗粒对总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）以及高密度脂蛋白胆固醇（HDL-C）未见明显影响（P〉0．05）。主动脉光镜下病理学观察，空白对照组：动脉壁结构清晰，内膜光滑，内皮细胞完整连续，内弹性膜及中膜弹力纤维形态正常，未见病理变化，平滑肌细胞排列规则。模型组：内膜明显增厚，内膜表面不光滑，弹力纤维断裂，平滑肌细胞排列紊乱，大量泡沫细胞形成，可见大块脂质侵润斑块向管腔凸出，大量增生的炎性细胞。血脂康组：动脉壁结构较清晰，内膜较光滑，有少量的内皮细胞泡沫样改变，平滑肌细胞排列较规则，增生的炎性细胞减少。清脂通脉颗粒低剂量组：大部分结构清晰，内膜较光滑，偶见主动脉内皮细胞泡沫样改变，平滑肌细胞排列较规则，增生的炎性细胞减少。清脂通脉颗粒中剂量组：主动内膜较光滑，偶见少量轻微的内皮细胞泡沫样改变，平滑肌细胞排列较规则，炎性细胞减少。清脂通脉颗粒高剂量组：主动脉结构清晰，内膜较光滑，内皮细胞泡沫样改变消失，平滑肌细胞排列规则，炎性细胞明显减少。结论]清脂通脉颗粒能降低血脂，抗动脉粥样硬化机制可能与降脂有关。

Qingzhi Tongmai Particles to Improve Blood Lipids and Rabbit Anti-atherosclerosis Mechanism Randomized Controlled Study

Objective ] Explore Qingzhi Tongmai lipid particles in rabbit aortic atherosclerosis and its mechanisms. Methods ] Fat rabbit model established model. The 48 rabbits were randomly divided into 6 groups randomly ： control group , model group, Xuezhikang group, Qingzhi Tongmai particles low dose group, Qingzhi Tongmai particles dose group , QingZhi Tongmai particles high-dose groups of eight . After the modeling , in addition to the blank control group, the other groups according to the following dose gavage , 15mL/kg / time, 2 times / d, the end of the experimental period to 10 weekends . Model group, normal saline ; Xuezhikang I Xuezhikang 0.1g/kg ~ d; Qingzhi Tongmai particles low-dose group , middle dose group and high dose group were I Qingzhi Tongmai for the sample of particles at doses of 0.69g/kg · d （ 3g crude drug / kg / d ）, 2.31g/kg · d （ 10g crude drug / kg / d ） and 6.94g/kg· d （ 30g crude drug / kg / d ） . Treatment for 10 weeks. After treatment, the serum triglyceride determination （ TG ）, total cholesterol （ TC ）, high density lipoprotein cholesterol （ HDL-C ）, low density lipoprotein cholesterol （ LDL-C ） . Result ] Serum values of model group 4 test results were higher than the control group （ P〈0.05 ）, the model was established that high blood lipids. Qingzhi Tongmai particles, the high-dose group triglyceride （ TG ） were significantly lower than the model group （ P〈0.05 ）, described Qingzhi Tongmai particles can reduce high blood cholesterol rabbit serum triglycerides, and experiment with Xuezhikang dose effect similar （ P〉O.05 ） . Qingzhi Tongmai particles on total cholesterol （ TC ）, low density lipoprotein cholesterol （ LDL-C ） and high-density lipoprotein cholesterol （ HDL-C ） had no significant effect （ P〉O.05 ） . Aortic pathology observed under light microscope, blank control group ： the arterial wall structure is clear , intimal smooth, continuous endothelial cell integrity , internal elastic membrane and the membrane elastic fibers were normal , no pathological changes in smooth muscle cells arranged regularly . Model group： intimal thickening , intimal surface is not smooth , elastic fiber breakage , smooth muscle cell disorder , a lot of foam cell formation , showing large lipid infiltration plaque protruding into the lumen , inflammatory ceils proliferated. Xuezhikang ： arterial wall structure clearer , intimal smooth, a small amount of foam -like changes in endothelial cells , smooth muscle cells arranged in a more regular , reduced proliferation of inflammatory cells . Qingzhi Tongmai particles low-dose group ： Most of the clear structure , intimal smooth, occasionally foamy alter aortic endothelial ceils , smooth muscle cells arranged in a more regular , the proliferation of inflammatory cells decreased. Qingzhi Tongmai particles dose group ： Active intimal smooth, occasionally a small amount of foamy slight changes in endothelial cells , smooth muscle ceils arranged in a more regular , inflammatory cells decreased. Qingzhi Tongmai particles high dose group ： clear structure aortic intimal smooth, like changes in endothelial cell foam disappears , smooth muscle cells arranged rules , inflammatory cells decreased significantly. Conclusion ] Qingzhi Tongmai particles can reduce blood lipids, and its anti- atherosclerotic mechanisms may be related to lipid-lowering effects.