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电针对大鼠局灶脑缺血再灌注后脑内血管生长因子和血管抑制因子表达的影响
引用本文:马璟曦,罗勇.电针对大鼠局灶脑缺血再灌注后脑内血管生长因子和血管抑制因子表达的影响[J].中国针灸,2007,27(2):129-133.
作者姓名:马璟曦  罗勇
作者单位:重庆医科大学附属第一医院神经内科,重庆市神经病学重点实验室,重庆,400016
基金项目:重庆市科委资助项目;重庆市卫生局资助项目
摘    要:目的:探讨电针对缺血性脑卒中的作用机制。方法:线栓法制备Wistar大鼠局灶脑缺血再灌注模型。将大鼠随机分为正常组、模型组、电针组。选取双侧“合谷”穴为电针穴位,采用原位杂交法检测血管内皮生长因子(VEGF)mRNA的表达,免疫组化法检测血管生成素(Ang-1)和内皮抑素(Endostatin)蛋白的表达。结果:模型组VEGFmRNA、Ang-1蛋白、Endostatin蛋白的表达较正常组增加(P<0·05),电针组VEGFmRNA、Ang-1蛋白比模型组增加更明显(P<0·05),而En-dostatin蛋白表达增加较模型组明显下降,差异有显著性意义(P<0·05)。结论:电针可能通过上调血管生长因子和下调血管抑制因子的表达来促进局灶脑缺血再灌注后的血管新生。

关 键 词:电针  再灌注损伤/针灸疗法  血管内皮生长因子类/针灸效应  基因表达/针灸效应
文章编号:0255-2930(2007)02-0129-05
修稿时间:2006年2月5日

Effects of electroacupuncture on expression of angiogenic growth factors and antiangiogenic growth factors in the brain tissue of the rat after focal cerebral ischemia reperfusion
MA Jing-xi,LUO Yong.Effects of electroacupuncture on expression of angiogenic growth factors and antiangiogenic growth factors in the brain tissue of the rat after focal cerebral ischemia reperfusion[J].Chinese Acupuncture & Moxibustion,2007,27(2):129-133.
Authors:MA Jing-xi  LUO Yong
Institution:Department of Neurology, The First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing Key Laboratory of Neurology, Chongqing 400016, China.
Abstract:OBJECTIVE: To explore the mechanism of electroacupuncture (EA) in improving ischemic stroke. METHODS: A Wistar rat model of focal cerebral ischemia reperfusion was made by filament occlusion. The rats were randomly divided into a normal group, a model group, an EA group. EA was given at bilateral "Hegu" (LI 4) in the EA group. Vascular endothelial growth factor (VEGF) mRNA was detected with in situ hybridization and expression of angiogenin-1 (Ang-1) and endostatin proteins with immunohistochemical method. RESULTS: The expressions of angiogenic growth factors including VEGF and Ang-1 in the EA group were significantly increased, while the expressions of endostatin was significantly decreased as compared with those in the model group (both P<0.05). CONCLUSION: EA improving ischemic stroke is carried out possibly through up-regulating the expression of angiogenic growth factors and down-regulating the expression of antiangiogenic growth factors.
Keywords:Electroacupuncture  Reperfusion Injury/am ther  Vascular Endothelial Growth Factors/am eff  Gene Expression/am eff
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