针刺对快速老化模型鼠脑中衰老相关基因表达的调整作用

目的：探讨针刺调整作用在分子水平的反映。方法：以快速老化模型鼠（SAM）脑中衰老相关基因的表达为研究对象。应用由磁珠分离法纯化mRNA,SMART-PCRcDNA合成和AFLP-银染法结合形成的mRNA-AFLP分析技术，进行mRNA指纹检测，寻找衰老相关性差异表达的基因片段。并观察针刺对其表达水平的影响。结果：随机应用20对引物作选择性AFLP扩增，共发现42条带型较为清晰的SAMP，R两系组间差异表达带，其中，33条（约占78.6%)在P系相对于R系低表达，9条（约占21.4%)在P系相对于R系高表达，针刺SAMP10的不同腧穴，可使异常低表达的基因表达水平不同程度地上调，使异常高表达的基因表达水平不同程度地下调，其变化均趋同于正常老化的SAMR1。结论：针刺对衰老机体紊乱的分子网络能发挥整体调节作用。促使其恢复协调平衡，从而延缓衰老进程。

Regulatory action of acupuncture on expression of aging-correlative genes in the brain of the senescence accelerated mouse

Objective To explore regulatory action of acupuncture at molecular level through the study on expression of aging-correlative genes in the senescence accelerated mouse (SAM). Methods mRNA was purified with magnetic bead separation method, mRNA-AFLP analysis technique formed by combination of SMA RT-PCR cDNA synthesis and AFLP-silver staining method were used for fingerprinting detection, to search for gene segments of aging-correlative differential expression and investigate effect of acupuncture on the expression level. Results 20 pairs of primer were randomly applied as selective AFLP amplification, 42 clear differential expression bands between SAM P and R serieses were found, among them, 33 bands (about 78.6%) in P series were lower expression related to R series, and 9 (about 21.4%) in the P series were higher expression related to the R series. Acupuncture at different acupoints in the SAM P10 could up-regulate the gene expression level of the abnormally low expression genes and down-regulate the gene expression level of the abnormally high expression genes in varying degrees. All the changes tended to the normal aging SAMR1. Conclusion Acupuncture can regulate the disordering molecular networks in aging organisms at whole, promoting recovery of balance, hence postponement of aging process.