针灸"肾俞"穴对吗啡依赖大鼠戒断反应和成瘾脑区神经元活动的影响

目的:探讨电针肾俞穴对吗啡戒断的影响及其作用机制.方法:建立急性吗啡成瘾模型,采用瞬时基因c-fos表达作为神经元活动的标志,运用免疫组化技术,对电针"肾俞"穴对抗吗啡戒断症状以及可能涉及的脑区进行研究.结果:电针"肾俞"穴能明显抑制大鼠戒断症状,降低导水管周围灰质区、下丘脑室旁核、海马CA1区、CA3区和齿状回和下丘脑外侧区的c-fos表达,但增加杏仁基底外侧核、杏仁中央核和伏隔核核区(core)的c-fos表达.结论:电针"肾俞"穴能有效抑制吗啡戒断症状,电针对抗阿片类药物依赖涉及多个中枢核团.

Effects of acupuncture at Shenshu (BL 23)on morphine withdrawal syndrome and c-fos expression in addiction-relative brain regions

Objective To probe the effect of electroacupuncture (EA) at Shenshu (BL 23) on morphine withdrawal syndrome and the mechanism. Methods Morphine dependence was induced in the rat by subcutaneous injection of morphine, and the acute withdrawal syndrome was precipitated b y intraperitoneal administration of naloxone. The rats were pretreated with 100 H z EA at Shenshu (BL 23) for one hour before the naloxone challenge. Withdrawal scores were calculated, and c-fos levels were detected by immunocytochemical meth od. Results EA at Shenshu (BL 23) could obviously inhibit withdrawal syndrome, decreased expression of c-fos in the PAG, paraventricular hypothalamus nucleus, lateral hypothalamic area and hypocampus while EA increase c-fos positive neurons in the amygdula and core of nucleus accumbens. Conclu sion EA at Shenshu (BL 23) can effectively inhibit morphine withdrawal s yndrome and many central nucleus groups are involved in the inhibition of EA on opioid m edicine dependence.