水沟穴最佳刺激参数的筛选研究

目的:了解针刺参数对针效的影响,探讨"醒脑开窍"针刺法中水沟穴最佳刺激参数。方法:复制大鼠大脑中动脉缺血模型(MCAO),以针刺频率的慢、中、快(1、2、3次/秒)和针刺时间的短、中、长(5 s、60 s、180 s)的两因素三水平针刺参数搭配组合9种针刺方法刺激"水沟"穴,以脑血流量为效应指标,通过正交试验、析因分析、单因素方差分析,综合分析不同针刺手法作用于"水沟"穴对MCAO大鼠脑血流量的影响。结果:针刺效应受针刺频率、时间以及它们两者的交互作用影响,其中,针刺时间影响作用最大。尽可能长时间地应用最快频率针刺,可取得最佳针刺效应。结论:"醒脑开窍"针刺法中"水沟"穴的操作是快频率、足够长时间的针刺(雀啄手法以眼球湿润为度),可取得最佳效应。此法是"水沟"穴最佳刺激参数。

Study on optimum stimulation parameter for Shuigou (GV 26) in Xingnao Kaiqiao needling method

Objective To investigate the influence of acupuncture parameter on acupuncture effect and to probe the optimum stimulation parameter for Shuigou (GV 26) in Xingnao Kaiqiao needling method.Methods The rat model of middle cerebral artery obstruction (MCAO) was replicated and"Shuigou"(GV 26) was stimulated by 9 acu- puncture parameters combined by two factors at 3 levels,i.e.frequency 1,2,3 times/second,and needling dura- tion 5 s,60 s,180 s.The effects of acupuncture at"Shuigou"(GV 26) with the 9 different acupuncture parameters on cerebral blood flow (CBF) in the MCAO rat were investigated by orthogonal test,factor analysis and one-way ANOVA.Results Acupuncture effect was influenced by acupuncture frequency,acupuncture duration and their combination.Among them,the acupuncture duration was the most important factor.The best acupuncture effect could be attained by using as long acupuncture duration as possibly with the fastest acupuncture frequency.Conclu- sion In the Xingnao Kaiqiao needling method,acupuncture at"Shuigou"(GV 26) with fastest frequency and enough long duration can get the best acupuncture effect,which are the optimum stimulation parameters for"Shui- gou"(GV 26).