咳喘宁对支气管哮喘大鼠肺组织一氧化氮和内皮素-1含量的影响

目的：观察咳喘宁对支气管哮喘大鼠肺组织一氧化氮（NO）和内皮素（ET-1）含量的影响。方法：40只SD大鼠随机分为正常组、模型组、咳喘宁高剂量组（27g生药／kg体质量）、咳喘宁低剂量组（13．5g生药／kg体质量）、桂龙咳喘宁组（0．41g／kg体质量），每组8只。除正常组外以卵蛋白致敏并吸入激发法制备大鼠支气管哮喘模型，各治疗组均从第1次哮喘激发开始（造模第3周）至处死前每天灌胃给药，激发并给药4周后处死大鼠，观察肺组织NO、ET-1含量和病理组织学变化。结果：与正常组比较，模型组大鼠支气管管壁和平滑肌厚度、嗜酸性粒细胞、淋巴细胞数以及肺组织NO、ET-1含量明显增加（P〈0．01）；与模型组比较，各治疗组均可显著降低支气管管壁和平滑肌厚度、嗜酸性粒细胞、淋巴细胞数以及肺组织NO、ET-1含量（P〈0．05或P〈0．01）；且咳喘宁高、低剂量组优于桂龙咳喘宁胶囊组（P〈0．05或P〈0．01）。结论：咳喘宁可通过降低肺组织NO和ET-1含量，抑制支气管哮喘大鼠气道炎症和气道重塑等病理过程。

Effects of Kechuanning on NO and ET-1 in Lung Tissue of Rat with Bronchial Asthma

Objective: To observe the effects of Kechuanning on nitrogen monoxidum(NO) and endothelin(ET-1) of in pulmonary tissue of rat with bronchial asthma. Methods: 40 rats were randomly divided into 5 groups: the normal group,the model group,Kechuanning high-dose group(27g/kg),Kechuanning low-dose group (13.5g/kg) and Guilong Kechuanning Capsule control group(0.41g/kg),8 rats each group.The bronchial asthmatic model was established by egg protein sensibilization and long-term inhalation provocation.The rats of each treatment groups were given intragastric administration each day from the first time of provocation to execution.After 4 weeks of treatment,the rats were killed and lung tissue were taken to dyeing of HE to be observed.The contents of NO and ET-1 in lung tissue were determined.Results: Compared with the normal group,the thickness of bronchus wall and bronchus smooth muscle,the numbers of eosinophile granulocyte and leukomonocyte,and the contents of NO and ET-1 in lung tissue were increased in the model group(P(<0.01));Compared with the model group,the thickness of bronchus wall and bronchus smooth muscle,the numbers of eosinophile granulocyte and leukomonocyte and the contents of NO and ET-1 in lung tissue were decreased in the each treatment group(P<0.05 or P<0.01);And Kechuanning high-dose and low-dose groups surpass Guilong Kechuanning Capsule group(P<(0.05) or P<0.01).Conclusion: Kechuanning could decrease the contents of NO and ET-1 in lung tissue,so as to restrain air passage inflammation and against airway remolding.