| 正交实验法优选黄连-厚朴药对的提取工艺 |
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| 引用本文： | 吴安国,曾宝,韦杨,李生梅,黄孟秋,黄斌,陈建南,赖小平. 正交实验法优选黄连-厚朴药对的提取工艺[J]. 中华中医药杂志, 2012, 0(1): 104-107 |
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| 作者姓名： | 吴安国 曾宝 韦杨 李生梅 黄孟秋 黄斌 陈建南 赖小平 |
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| 作者单位： | 广州中医药大学新药开发研究中心;东莞广州中医药大学中医药数理工程研究院 |
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| 基金项目： | 国家科技支撑计划项目(No.2006BAI11B00)~~ |
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| 摘 要： | 目的:优选黄连-厚朴药对的提取工艺。方法:以小檗碱与厚朴总酚的提取率以及出膏率为指标,采用正交实验法,考察溶剂量,提取次数,提取时间,醇浓度对各指标的影响。运用高效液相色谱法,测定小檗碱,厚朴总酚的含量,流动相:乙腈:0.3%磷酸(三乙胺调节pH=4.0)。结果:黄连厚朴的最佳提取工艺为10倍量药材,70%乙醇回流,提取60min,提取1次。结论:该工艺稳定,可靠,省时,省材。为黄连-厚朴配伍的复方制剂的工艺研究提供了实验依据。
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| 关 键 词： | 黄连 厚朴 提取工艺 正交实验法 高效液相色谱法 |
Optimize the extraction technology of the couplet medicines Coptis-Magnolia by orthogonal |
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| WU An-guo,ZENG Bao,WEI Yang,LI Sheng-mei,HUANG Meng-qiu,HUANG Bin,CHEN Jian-nan,LAI Xiao-ping. Optimize the extraction technology of the couplet medicines Coptis-Magnolia by orthogonal[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2012, 0(1): 104-107 |
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| Authors: | WU An-guo ZENG Bao WEI Yang LI Sheng-mei HUANG Meng-qiu HUANG Bin CHEN Jian-nan LAI Xiao-ping |
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| Affiliation: | WU An-guo1,2,ZENG Bao1,2,WEI Yang1,LI Sheng-mei1,HUANG Meng-qiu1,HUANG Bin1,CHEN Jian-nan1,LAI Xiao-ping1,2(1Research and Development Centre of New Drugs,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;2Dongguan Mathematical Engineering Academy of Chinese Medicine,Guangzhou University of Chinese Medicine,Dongguan 523808,China) |
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| Abstract: | Objective: To optimize the extraction process of the couplet medicines Coptis-Magnolia.Methods: Amount of solvent,the extraction time and number of extraction,alcohol concentration on effect of the extraction rate of berberine and the total phenol,the paste rate were evaluated by orthogonal.Determinate the content of berberine and the total phenol by using high performance liquid chromatography(HPLC),the mobile phase: acetonitrile: 0.3% phosphate(adjusting pH to 4 with triethylamine).Results: The best extraction process of the compatibility of Coptis-Magnolia is that they are extracted for 60min with 70% ethanol which is 10 times as much as medicine,and extracting one time.Conclusion: The extraction process is stable,reliable,saving time and materials.The study providing the experimental evidence for the extraction process of couplet medicines Coptis-Magnolia |
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| Keywords: | Coptis Magnolia Extraction technology Orthogonal HPLC |
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