| 高效液相色谱法测定三黄散瘀巴布剂中盐酸小檗碱的含量 |
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| 引用本文： | 王知青,尹华,许丛辉,章建华,刘云飞.高效液相色谱法测定三黄散瘀巴布剂中盐酸小檗碱的含量[J].中华中医药杂志,2011(11):2690-2692. |
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| 作者姓名： | 王知青 尹华 许丛辉 章建华 刘云飞 |
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| 作者单位： | 浙江中医药大学药学院中药标准化研究实验室;浙江中医药大学附属第一医院; |
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| 基金项目： | 浙江省科技厅科技计划项目(No.2007C23020); 浙江省中药现代化专项资金项目(No.浙财企字[2008]268号); 浙江省中医药管理局项目(No.2006C038)~~ |
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| 摘 要： | 目的:建立三黄散瘀巴布剂中盐酸小檗碱的HPLC含量测定方法。方法:HPLC法,色谱柱:Agilent Zorbax Eclipse XDB C8(150mm×4.6mm,5μm);流动相:乙腈-0.1%磷酸溶液:50:50(每100mL流动相加入十二烷基硫酸钠0.1g);检测波长:265nm;流速:1mL/min,柱温:25℃;进样量:10μL。结果:盐酸小檗碱在41.2-772.5ng的范围内线性关系良好,回归方程为:Y=2.344X-32.42(r=0.9997);加样回收率在96.91%-102.00%,平均回收率为99.70%,RSD为2.0%。结论:该方法简便快速、结果准确可靠、重复性好,可用于三黄散瘀巴布剂的质量控制。
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| 关 键 词： | 三黄散瘀巴布剂 盐酸小檗碱 高效液相色谱 含量测定 |
Determination of Berberine Hydrochloride in Sanhuang Sanyu Cataplasm by HPLC |
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| WANG Zhi-qing,YIN Hua,XU Cong-hui,ZHANG Jian-hua,LIU Yun-fei.Determination of Berberine Hydrochloride in Sanhuang Sanyu Cataplasm by HPLC[J].China Journal of Traditional Chinese Medicine and Pharmacy,2011(11):2690-2692. |
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| Authors: | WANG Zhi-qing YIN Hua XU Cong-hui ZHANG Jian-hua LIU Yun-fei |
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| Institution: | WANG Zhi-qing1,YIN Hua1,XU Cong-hui1,ZHANG Jian-hua2,LIU Yun-fei1(1Research Laboratory for Standardization of Chinese Medicines,College of Pharmacy,Zhejiang Chinese Medicine University,Hangzhou 310053,China,2The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310006,China) |
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| Abstract: | Objective: To establish a content determination method of Berberine hydrochloride in Sanhuang Sanyu Cataplasm by HPLC.Methods: RP-HPLC was performed to determine Berberine hydrochloride on a column of Agilent Zorbax Eclipse XDB-C8(150mm×4.6mm,5μm) at 25℃;Acetonitrile-0.1% H3PO4(50:50)(Adding 0.1g C12H25SO4Na to every 100mL) was used as mobile phase,the flow rate was 1.0mL/min.The detection wavelength was at 265nm.Results: A good linearity can be found in the range of 41.2-772.5ng,and the regression equation was Y= 2.344X-32.42(r=0.9997).The recovery was between 96.91%-102.0%,and the average recovery rate was 99.70%,RSD was 2.0%.Conclusion: The method was simple,rapid,accurate and available,which can be used to control the quality of Sanhuang Sanyu Cataplasm. |
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| Keywords: | Sanhuang Sanyu Cataplasm Berberine hydrochloride HPLC Content determination |
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