| 黄芪对2型糖尿病外周血内皮祖细胞增殖、分化的影响 |
| |
| 引用本文： | 徐寒松,雷闽湘,孔德明,向慧,谢晓云.黄芪对2型糖尿病外周血内皮祖细胞增殖、分化的影响[J].中华中医药杂志,2009(2). |
| |
| 作者姓名： | 徐寒松 雷闽湘 孔德明 向慧 谢晓云 |
| |
| 作者单位： | 中南大学湘雅医院内分泌科;贵阳中医学院第二附属医院内分泌科;中南大学湘雅二医院; |
| |
| 基金项目： | 贵州省科技厅科学技术基金资助课题(No.黔科合J字[2006]2092号) |
| |
| 摘 要： | 目的:研究黄芪对2型糖尿病(T2DM)患者外周血内皮祖细胞(endothelial progenitor cells,EPCs)增殖、分化的影响。方法:选择T2DM患者和非T2DM对照人群各20例,密度梯度离心法获取外周血单个核细胞,培养7d后,收集贴壁细胞并加入黄芪注射液(0.2,2,20,200mg/ml)干预一定时间(6h,12h,24h,48h)。激光共聚焦显微镜鉴定EPCs,MTT法、流式细胞仪检测黄芪对T2DM患者EPCs增殖、分化的影响。结果:T2DM患者外周血EPCs数量减少(P<0.01),增殖能力受损(P<0.05),EPCs数量、增殖能力与糖化血红蛋白(HbA1c)水平和糖尿病病程年呈负相关(P<0.01);T2DM患者外周血EPCs CD14+、CD64+细胞百分比明显高于对照组,而vWF+细胞百分比明显低于对照组(P<0.01)。黄芪对T2DM患者外周血EPCs具有明显的促增殖及促进其向内皮细胞系分化的作用。结论:T2DM患者外周血EPCs数量减少、增殖及分化能力受损;黄芪对T2DM患者外周血EPCs增殖及向内皮细胞系分化有显著促进作用。
|
| 关 键 词： | 黄芪 2型糖尿病 内皮祖细胞 增殖 分化 |
Effect of Astragalus membranaceus on proliferation,differentiation of endothelial progenitor cells from peripheral blood in patients with type 2 diabetes |
| |
| XU Han-song,LEI Min-xiang,KONG De-ming,XIANG Hui,XIE Xiao-yun.Effect of Astragalus membranaceus on proliferation,differentiation of endothelial progenitor cells from peripheral blood in patients with type 2 diabetes[J].China Journal of Traditional Chinese Medicine and Pharmacy,2009(2). |
| |
| Authors: | XU Han-song LEI Min-xiang KONG De-ming XIANG Hui XIE Xiao-yun |
| |
| Institution: | XU Han-song1,2,LEI Min-xiang1,KONG De-ming2,XIANG Hui3,XIE Xiao-yun1(1Department of Endocrinology,Xiangya Hospital,Central South University,Changsha 410008,China,2Department of Endocrinology,the Second Affiliated Hoapital of Guiyang College of TCM,Guiyang 550003,3No. 2Xiangya Hospital of Central South University,Changsha 410011,China) |
| |
| Abstract: | Objective:To study the effects of Astragalus membranaceus on the proliferation,differentiation of endothelial progenitor cells(EPCs) from peripheral blood in patients with type 2 diabetes. Methods:Total mononuclear cells were isolated from patients with type 2 diabetes(n=20) and age-matched control subjects(n=20) by Ficoll density gradient centrifugation,and then the cells were plated on fibronectin coated culture dishes. After 7 days of culture,attached cells were stimulated with Astragalus membranaceus in... |
| |
| Keywords: | Astragalus membranaceus Type 2 diabetes Endothelial progenitor cell Proliferation Differentiation |
| 本文献已被 CNKI 等数据库收录！ |
|
