| 丹酚酸A对肝癌HepG2细胞线粒体跨膜电位的影响 |
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| 引用本文： | 毕蕾,陈卫平,姜泽群,冯全服.丹酚酸A对肝癌HepG2细胞线粒体跨膜电位的影响[J].中华中医药杂志,2012(4):1171-1173. |
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| 作者姓名： | 毕蕾 陈卫平 姜泽群 冯全服 |
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| 作者单位： | 南京中医药大学附属医院老年医学科;南京中医药大学基础医学院 |
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| 基金项目： | 国家自然科学基金(No.81072777);江苏省高校优势学科建设工程资助项目;“十一五”国家科技支撑计划项目(No.2006BAI11B08)~~ |
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| 摘 要： | 目的:通过研究丹酚酸A(SalA)对肝癌HepG2细胞株线粒体跨膜电位的影响,来探讨SalA抑制肝癌细胞增殖的作用机制。方法:以肝癌HepG2细胞为靶细胞,采用MTT法观察不同浓度的SalA对HepG2细胞增殖的影响;通过Hoechst和Mito-tracke双染,经Thermo Cellomics ArrayScan VTi检测SalA对HepG2细胞的线粒体跨膜电位和细胞毒性的影响。结果:SalA对HepG-2细胞增殖有抑制作用,并呈现剂量依赖性;SalA能降低HepG2细胞内线粒体跨膜电位水平,且随浓度增加降低越明显。结论:SalA具有抑制肝癌HepG2细胞增殖的作用,其作用机制可能与降低线粒体跨膜电位,通过线粒体途径触发细胞凋亡有关。
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| 关 键 词： | 丹酚酸A HepG2 线粒体 跨膜电位 细胞增殖 |
Effect of Salvianolic acid A on mitochondrial transmembrane potential of human hepatocellular carcinoma cell line HepG2 |
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| BI Lei,CHEN Wei-ping,JIANG Ze-qun,FENG Quan-fu.Effect of Salvianolic acid A on mitochondrial transmembrane potential of human hepatocellular carcinoma cell line HepG2[J].China Journal of Traditional Chinese Medicine and Pharmacy,2012(4):1171-1173. |
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| Authors: | BI Lei CHEN Wei-ping JIANG Ze-qun FENG Quan-fu |
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| Institution: | 1Geriatrics Department,The Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China;2 Basic Medical School of Nanjing University of Chinese Medicine,Nanjing 210046,China) |
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| Abstract: | Objective: Study the effect of Salvianolic acid A(SalA) on mitochondrial transmembrane potential of human hepatocellular carcinoma cell line HepG2,to explore the mechanism of SalA in vitro inhibition of hepatocellular carcinoma cell.Methods: Hepatocellular carcinoma cell line HepG2 were used as target cells,and MTT method was used to observe the effects of different concentrations of SalA on HepG2 cells.Thermo Cellomics ArrayScan VTi was used for detecting the effect of mitochondrial transmembrane potential and cytotoxicity of Salvianolic acid A(SalA) on HepG2 cells by Hoechst and Mito-tracke double staining.Results: The growth of HepG-2 cells was inhibited by SalA,and show a certain degree of dose dependent.Through the Thermo Cellomics ArrayScan VTi testing found that SalA can reduce the mitochondrial transmembrane potential levels of HepG-2 cells,and decreased more obviously with the increasing concentration.Conclusion: SalA can inhibit HepG2 cells.Its mechanism may be decreasing mitochondrial transmembrane potential,and triggering the cells apoptosis. |
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| Keywords: | Salvianolic acid A HepG2 Mitochondrial Transmembrane potential Cell proliferation |
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