慢性再生障碍性贫血中医辨证分型与Fas/FasL抗原表达相关性研究

目的探讨慢性再生障碍性贫血中医辨证分型与Fas、FasL抗原表达的相关性,解析慢性再生障碍性贫血辨证分型的物质基础。方法选择90例慢性再生障碍性贫血患者,其中脾肾阳虚、肾阴阳两虚、肝肾阴虚者各30例;另设15例健康志愿者为正常对照组。应用流式细胞术检测慢性再生障碍性贫血患者和正常人骨髓Fas、FasL抗原表达率;逆转录聚合酶链反应方法(RT-PCR)检测骨髓单个核细胞Fas、FasL mRNA表达。结果慢性再生障碍性贫血患者Fas抗原、FasL抗原的阳性率、mRNA表达均高于正常对照组(P<0.05或P<0.01),而且脾肾阳虚组、肾阴阳两虚组与肝肾阴虚组组间Fas抗原、FasL抗原的阳性率、mRNA表达存在明显差异(P<0.05或P<0.01),按脾肾阳虚组、肾阴阳两虚组与肝肾阴虚组次序依次升高。结论慢性再生障碍性贫血的辨证分型与Fas、FasL抗原表达存在相关性,可作为慢性再生障碍性贫血辨证分型的物质基础和定量表征。

Correlative Study on Chinese Medical Syndromes and Fas/FasL Antigen Expression of Chronic Aplastic Anemia

Objective To study the correlation between Chinese medical syndromes and Fas, FasL antigen expressions of chronic aplastic anemia and analyze the material basis of its syndrome differentiation. Methods Detect Fas and FasL antigen expression rate in the bone marrow of CAA by flow cytometry and use RT-PCR to assay Fas and FasL mRNA with bone marrow mononuclear cell. Results Fas and FasL antigen expression rate of bone marrow and Fas, FasL mRNA expression was higher than control group(P < 0.05, P < 0.01); compared with these three syndromes, Fas and FasL expression of liver-kidney yin deficiency was the highest; kidney yin-yang deficiency came next, and spleen-kidney yang deficiency lowest, with significant differences(P < 0.05, P < 0.01). Conclusion Chinese medical syndrome is in close correlation with Fas and FasL antigen expression of chronic aplastic anemia, which can be represented as the substantial basis and the quantitative exosyndrome of the syndrome differentiation of CAA.