6种胃溃疡小鼠模型的证候特征及比较

目的研究胃溃疡不同模型小鼠证候的发生和演变,以及疾病与证候的关系。方法在文献报道的基础上,采用水浸拘束法、乙酸法、磷酸组胺法、消炎痛法、利血平法、乙醇法等6种不同方法建立小鼠胃溃疡疾病模型,并设置正常、禁食、假手术等对照组,运用业已建立的小鼠标准化、计量化的四诊检测和辨证方法,对以上各模型进行了客观、动态、量化的检测和证候评价。结果6种造模方法均能不同程度地造成胃黏膜损伤。其中,水浸拘束模型气血阴阳损伤程度轻,恢复快;乙酸法模型出现气损、阳竭,以及精血虚少的证候;磷酸组胺小鼠仅在早期有胃热证的倾向;消炎痛模型出现短暂气虚、阳虚证候,但精血损伤证候持续较长;利血平模型出现典型的胃寒证、阳虚证(主要是心阳虚、脾阳虚)和阴虚,是一个严重的气血阴阳虚证复合模型;乙醇法造模后即出现典型的胃热证,恢复期甚至出现气盛表现。结论不同造模方法的胃溃疡模型小鼠存在各自的证候特点,符合"有是病便有是证"的中医理论;同时其证候是可以通过四诊客观化方法检测到,并可以用于治则治法制定的参考,以及作为疗效评价指标。

Syndrome Characteristics and Comparison of Six Kinds of Mice Gastrelceosis Models

Objective To describe the occurrence and evolution of the syndromes for different mice gastrelceosis models,as well as the relationship between diseases and syndromes.Methods Based on reports,we select six methods of stress,acetic acid,histamine phosphate,antifani,apoplon and alcohol to make gastric ulcer model simultaneously,and set up the normal,abrosia,and sham control groups.Then we use the standard and quantization mice four diagnostic and differentiation methods.Then we use the standard and quantization four diagnostic and differentiation methods for mice,which are established to examine and appraise the above models in an objective,dynamic and quantified way.Results All six kinds of methods impair the stomach mucous membrane to different degrees.Among them,in the water immersion model,impairment to qi and blood,yin and yang is slight and recovers quickly.There were qi-deficiency,yang exhaustion and blood and essence deficiency in the acetic acid model.The histamine phosphate model has the tendency of the syndrome of heat in the stomach only in the early period.The transient qi and yang deficiency syndrome is found in the antifani model,but blood and essence deficiency syndrome lasts longer.The apoplon model,with typical syndromes of cold in the stomach,typical yang-deficiency(mainly yang deficiency of heart and spleen) and yin deficiency,is a serious compound model of qi,blood,yin and yang deficiency.Typical syndrome of heat in the stomach appears soon after the alcohol model is made and abundance of qi was found on the recovery stage.Conclusion Different methods in making mice gastrelceosis models have respective syndrome characteristics.This tallies with the Chinese medicine theory:"where there is a disease,there is a syndrome".Simultaneously the syndrome characteristics can be detected by the four objective diagnostic methods and may be used as reference in formulating therapeutic principle and methods,as well as criteria for appraising curative effects.