| 重楼皂苷Ⅰ对肺癌循环肿瘤细胞凋亡及周期的影响 |
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| 引用本文： | 王青,阙祖俊,罗斌,郑婷婷,田建辉.重楼皂苷Ⅰ对肺癌循环肿瘤细胞凋亡及周期的影响[J].上海中医药杂志,2017,51(5):77-81. |
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| 作者姓名： | 王青 阙祖俊 罗斌 郑婷婷 田建辉 |
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| 作者单位： | 上海中医药大学附属龙华医院中医肿瘤研究所,上海,200032;上海市中医药研究院中医肿瘤研究所,上海,200032,上海中医药大学附属龙华医院中医肿瘤研究所,上海,200032;上海市中医药研究院中医肿瘤研究所,上海,200032,上海中医药大学附属龙华医院中医肿瘤研究所,上海,200032;上海市中医药研究院中医肿瘤研究所,上海,200032,上海中医药大学附属龙华医院中医肿瘤研究所,上海,200032;上海市中医药研究院中医肿瘤研究所,上海,200032,上海中医药大学附属龙华医院中医肿瘤研究所,上海,200032;上海市中医药研究院中医肿瘤研究所,上海,200032 |
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| 基金项目： | 国家自然科学基金项目(81373621)；上海中医药大学预算内项目(2015YSN37)；上海市卫生局中医药事业发展三年行动计划项目(ZY3-CCCX-2-1002) |
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| 摘 要： | 目的通过体外药效评价重楼皂苷Ⅰ对肺癌循环肿瘤细胞(circulating tumor cells,CTC)增殖、凋亡的影响。方法分别用不同浓度的重楼皂苷Ⅰ干预CTC细胞24 h、48 h、72 h后,采用CCK-8法检测细胞增殖情况,采用流式细胞术和激光共聚焦显微镜分析CTC凋亡与周期情况。结果重楼皂苷Ⅰ能够显著抑制CTC增殖并具有浓度依赖性,诱导CTC细胞核形态发生明显改变,使凋亡细胞的比例显著升高,并将CTC细胞的周期阻滞在G0/G1期。结论重楼皂苷Ⅰ通过诱导CTC凋亡,抑制细胞从G0/G1期向S期转化,从而发挥抗肿瘤作用。
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| 关 键 词： | 重楼皂苷Ⅰ 肺癌循环肿瘤细胞 细胞增殖 细胞凋亡 细胞周期 |
| 收稿时间： | 2017/3/2 0:00:00 |
Effects of Polyphyllin I on apoptosis and cycle of circulating tumor cells of lung cancer |
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| WANG Qing,QUE Zu-jun,LUO Bin,ZHENG Ting-ting and TIAN Jian-hui.Effects of Polyphyllin I on apoptosis and cycle of circulating tumor cells of lung cancer[J].Shanghai Journal of Traditional Chinese Medicine,2017,51(5):77-81. |
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| Authors: | WANG Qing QUE Zu-jun LUO Bin ZHENG Ting-ting and TIAN Jian-hui |
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| Institution: | Tumor Institute of Traditional Chinese Medicine,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai, 200032;Tumor Institute of Traditional Chinese Medicine,Shanghai Academy of Traditional Chinese Medicine,Shanghai,200032,Tumor Institute of Traditional Chinese Medicine,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai, 200032;Tumor Institute of Traditional Chinese Medicine,Shanghai Academy of Traditional Chinese Medicine,Shanghai,200032,Tumor Institute of Traditional Chinese Medicine,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai, 200032;Tumor Institute of Traditional Chinese Medicine,Shanghai Academy of Traditional Chinese Medicine,Shanghai,200032,Tumor Institute of Traditional Chinese Medicine,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai, 200032;Tumor Institute of Traditional Chinese Medicine,Shanghai Academy of Traditional Chinese Medicine,Shanghai,200032 and Tumor Institute of Traditional Chinese Medicine,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai, 200032;Tumor Institute of Traditional Chinese Medicine,Shanghai Academy of Traditional Chinese Medicine,Shanghai,200032 |
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| Abstract: | Objective:To evaluate the effects of Polyphyllin I on proliferation and apoptosis of circulating tumor cells (CTC) of lung cancer in vitro. MethodsThe CTC were intervened by Polyphyllin I at different concentrations for 24,8 and 72 hours. The cell proliferation was determined by CCK-8,and the cell apoptosis and cycle were detected by flow cytometry and confocal laser scanning microscopy. Results:Polyphyllin I could significantly inhibit the CTC proliferation in a concentration-dependent manner,and induce the significant morphological changes of CTC nucleus. The proportion of apoptotic cells was significantly increased,and the cells cycle was stagnated in G0/G1 phase. Conclusion:Polyphyllin I can induce CTC apoptosis and inhibit the cells differentiation from G0/G1 to S phase,which plays an anti-tumor effects. |
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