多因素AD动物模型的建立及针刺对其学习记忆功能的影响

为研究针刺对老年性痴呆（AD）的疗效，70只大鼠分成7组：正常对照组（A），模拟对照组（B），氯化铝（AlCl3)组（C），SCOP组（D），AlCl3 SCOP组（E），针刺组（F），药物组（G），除A，B两组外，其余各组大鼠腹腔注射D－半乳糖，在此基础上，予以相应处理：C组胃饲AlCl3,D组注射SCOP，E组胃饲AlCl3 注射SCOP，F，G组以E组法造模并分别予针刺（大椎，百会），喂服药物哈伯因，疗程30d,B组模拟E组，以生理盐水鼻饲和腹腔注射，A组不作任何处理，结果：C，D，E3组大鼠水迷宫游出时间，错误次数，跳台的触电潜伏期，3min错误次数，与B组比较均有改变，其中E组最为明显，F，G组的各项指标较E组改善，且两组间基本相同，提示：多因素损伤能更好地突出AD的特征：针刺能提高AD动物的学习记忆功能。

Establishment of Multi-factor AD Animal Model and Acupuncture Effect on Its Memory

To study the acupuncture effect on Alzheimer's disease(AD),seventy rats were randomized into 7 groups:group A(normal group),group B(control group),group C with AlCl 3,group D with ACOP,group E with AlCl 3 and ACOP,group F with acupuncture treatment and group G with medicine treatment.Except groups A and B,the rats in other groups were given abdominal injection of D-galactose and then treatment:group C with AlCl 3 by injection,group D with SCOP by perfusion,group E with AlCl 3 by perfusion and SCOP by injection,groups F and G modeled as group E and respectively treated by needling Dazhui,Baihui,and with Hupperzine A for 30 days;group B was treated with mormal saline by perfusion and abdominal injection,group A treated by nothing.Results:in comparison with group B,time taken to swim out of the water labyrinth,error frequency,electric latent period and 3-minute error frequency in groups C,D and E changed,especially in group E;the indexes in groups F and G improved more significantly than those in group E,but similar between group F and G.It is suggested that multi-factor injury can better reflect AD and acupuncture can improve the memory.