| 中药胃肠舒对Ca~(2+)信号传导途径的调控 |
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| 引用本文： | 宋晓冬,王美蓉,张瑾锦,刘文波.中药胃肠舒对Ca~(2+)信号传导途径的调控[J].中国中医基础医学杂志,2011(8). |
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| 作者姓名： | 宋晓冬 王美蓉 张瑾锦 刘文波 |
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| 作者单位： | 滨州医学院实验中心; |
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| 基金项目： | 国家自然科学基金资助项目(30672680 and 30873263); 烟台市科技攻关项目(2008162); 大学生科技创新计划(BY2007DKCX10 and BY2008DKCX21) |
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| 摘 要： | 目的:研究中药胃肠舒对Ca2+信号传导途径的调控。方法:肠推进实验和离体肠道平滑肌的收缩检测胃肠舒对肠道平滑肌的作用;免疫组化法检测肠组织NOS和ChAT的表达;CellTiter-GloTM法、流式细胞术和激光共聚焦显微镜分别检测ATP含量、线粒体跨膜电位和Ca2+浓度的变化。结果:胃肠舒组能提高大鼠卡红肠道推进率,使离体肠道平滑肌自发活动收缩幅度加大,降低了NOS的含量,增高了ChAT的表达,ATP、Ca2+及线粒体跨膜电位升高。结论:胃肠舒能兴奋肠道平滑肌加快肠蠕动,可能是通过Ca2+调控实现的。
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| 关 键 词： | 肠蠕动 一氧化氮合酶 胆碱乙酰化酶 三磷酸腺苷 跨膜电位 |
Regulatory effect of Wei Chang Shu on Ca~(2+) signaling transduction pathway |
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| SONG Xiao-dong,WANG Mei-rong,ZHANG Jin-jin,LIU Wen-bo.Regulatory effect of Wei Chang Shu on Ca~(2+) signaling transduction pathway[J].Chinese Journal of Basic Medicine In Traditional Chinese Medicine,2011(8). |
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| Authors: | SONG Xiao-dong WANG Mei-rong ZHANG Jin-jin LIU Wen-bo |
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| Institution: | SONG Xiao-dong,WANG Mei-rong,ZHANG Jin-jin,LIU Wen-bo(Experimental Center of Binzhou Medical College,Yantai 264003) |
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| Abstract: | Objective:To study the mechanism of Ca2+ signaling in the regulation of gastrointestinal motility propulsion by the Chinese medicine Wei Chang Shu.Methods:Effect of Wei Chang Shu was detected by the rat intestinal propulsion and contraction of the isolated intestinal smooth muscle.Effect of Wei Chang Shu on neurotransmitters was observed by the expression of Nitric oxide synthase and Choline acetylase with immunohistochemistry.Effect of Wei Chang Shu on kinetic energy was detected by ATP level,change of mit... |
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| Keywords: | Intestinal Motility Nitric Oxide synthase Choline acetylase Adenosine Triphosphate Transmembrane Potential |
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