冠心病血瘀证相关基因b13的筛查和临床验证

目的:筛查冠心病血瘀证病证结合证候相关基因。方法:临床区组选择符合诊断标准的冠心病血瘀证、冠心病非血瘀证、非冠心病血瘀证患者和正常健康者共40人,运用外周血mRNA差异显示获得差异条带、反向Northern法阳性验证、克隆测序,并进行生物信息学分析和临床验证。结果:得到的28条真实差异基因片段序列,与人类基因数据库中比对分析,获得的b13与人类基因淋巴细胞活化因子1有100%的同源性,在冠心病血瘀证组显著表达。结论:差异基因b13通过T淋巴细胞的活化,引起免疫反应,导致进一步内皮损伤,刺激内皮细胞表达细胞因子、趋化因子及细胞黏附分子,促进炎性细胞黏附及脂质沉积,参与了内皮损伤和动脉硬化的形成。与冠心病血瘀证的病理改变密切相关。

Selection and Clinical Validation of Relative Gene b13 of Blood Stasis Syndrome in Coronary Heart Disease

Objective: Screening the relevant genes of Combining Symptoms and Disease of the Blood Stasis Syndrome in Coronary Heart Disease.Methods:To choose 40 cases and divide them into groups of coronary heart disease,non-blood-stasis disease of coronary heart disease,blood-stasis diseases of non-coronary heart disease and healthy people,according to diagnostic criteria,and obtain their differential strips by the display of the difference of peripheral blood mRNA,their positive proof by reverse Northern Method and the sequence of their clones and analyze their bio-information and confirmation of clinic.Results:28 sequences of gene fragments with real difference are caught,Among them b13 are 100% homologous with signaling lymphocytic activation molecule family member 1 of human genes by through comparison and analysis in NCBI human genomic database,and was highly expressed in the Blood Stasis Syndrome in Coronary Heart Disease.Conclusion:b13 in the differential genes could arise out of immunoreaction by activating T-lymphocyte,and resulted in more damage of endothelium cells,then endothelium cells would secrete cytokines,chemokines and intercellular adhesion molecule(ICAM),and they would promote the conglutination of inflammatory cells and lipid sediment,lead to or participate in the formation of endothelial damage and arteriosclerosis,and was correlated closely with the pathological change of the blood-stasis diseases of coronary heart disease.