| 苏木、苏木+黄芪对荷瘤小鼠CD4~+ CD25~+调节性T细胞及相关调控分子的干预作用 |
| |
| 引用本文： | 于明薇,孙桂芝,祁鑫,李道睿,张培彤,吴洁.苏木、苏木+黄芪对荷瘤小鼠CD4~+ CD25~+调节性T细胞及相关调控分子的干预作用[J].中国中医基础医学杂志,2010(5). |
| |
| 作者姓名： | 于明薇 孙桂芝 祁鑫 李道睿 张培彤 吴洁 |
| |
| 作者单位： | 中国中医科学院广安门医院; |
| |
| 基金项目： | 国家自然科学基金资助项目(30672764) |
| |
| 摘 要： | 目的:观察苏木、苏木+黄芪对荷瘤小鼠脾CD4+CD25+调节性T细胞(Treg)表达及相关调控分子的干预作用。方法:复制C57BL/6小鼠Lewis肺癌动物模型,动态观察苏木、苏木+黄芪抑瘤率、肺转移抑制率,流式细胞术检测小鼠脾CD4+CD25+Treg表达,免疫磁珠分选小鼠脾CD4+CD25+细胞,RT-PCR检测小鼠脾CD4+CD25+细胞和瘤组织Foxp3、CTLA-4mRNA表达。结果:苏木组6d、苏木+黄芪组10d瘤重低于荷瘤对照组(P0.05);苏木+黄芪组20d肺转移抑制率为77.75%;15d苏木+黄芪组小鼠脾CD4+CD25+细胞表达低于荷瘤对照组(P0.05);苏木+黄芪组荷瘤小鼠瘤组织CTLA-4、Foxp3mRNA表达及脾CD4+CD25+分选后细胞Foxp3 mRNA表达低于荷瘤对照组(P0.05)。结论:益气活血中药组方苏木+黄芪可调节荷瘤小鼠脾Treg及相关调控分子表达,并且在淋巴组织和肿瘤微环境中都表现出作用。
|
| 关 键 词： | CD4+CD25+调节性T细胞 中药 肿瘤 免疫耐受 免疫磁珠分选 |
Expressions of CD4~+ and CD25~+ of Tumor-bearing mice and Its Regulatory Molecules Intervened by Caesalpinina Sappan and Caesalpinina Sappan matched Radix Astragali |
| |
| YU Ming-wei,SUN Gui-zhi,QI Xin,LI Dao-rui,ZHANG Pei-tong,WU Jie.Expressions of CD4~+ and CD25~+ of Tumor-bearing mice and Its Regulatory Molecules Intervened by Caesalpinina Sappan and Caesalpinina Sappan matched Radix Astragali[J].Chinese Journal of Basic Medicine In Traditional Chinese Medicine,2010(5). |
| |
| Authors: | YU Ming-wei SUN Gui-zhi QI Xin LI Dao-rui ZHANG Pei-tong WU Jie |
| |
| Institution: | YU Ming-wei,SUN Gui-zhi,QI Xin,LI Dao-rui,ZHANG Pei-tong,WU Jie(Guang Anmen hospital China Academy of TCM,Beijing 100053,China) |
| |
| Abstract: | Objective:To observe the expression of CD4 + CD25 + regulatory T cells(Treg)in spleen of tumor-bearing mice and its regulatory molecules intervened by Caesalpinina sappan(Sumu,SM) and Caesalpinina sappan + Radix Astragali(Sumu + Huangqi,SMHQ).Methods:Lewis lung cancer model in mice was established and treated with Caesalpinina sappan and Caesalpinina sappan + Radix Astragali.The tumor inhibiting rate and the inhibiting rate of lung metastasis were dynamically observed and the expression of CD4 + CD25 + Treg... |
| |
| Keywords: | CD4 + CD25 + regulatory T cells Chinese medicine herbs tumor immune tolerance MACS |
| 本文献已被 CNKI 等数据库收录！ |
|
