| 补肾方、柔肝方与化痰方对膝骨关节炎滑膜细胞增殖及COMP表达的影响 |
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| 引用本文： | 张鹏,石关桐,郑昱新,曹月龙.补肾方、柔肝方与化痰方对膝骨关节炎滑膜细胞增殖及COMP表达的影响[J].中国中医骨伤科杂志,2008,16(12):18-21. |
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| 作者姓名： | 张鹏 石关桐 郑昱新 曹月龙 |
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| 作者单位： | [1]上海市第六人民医院针推伤科,上海200233 [2]上海中医药大学附属曙光医院骨伤科,上海200233 |
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| 基金项目： | 国家自然科学基金资助项目项目号:30472222 |
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| 摘 要： | 目的：探讨补肾方、柔肝方和化痰方对膝骨关节炎滑膜细胞增殖及COMP表达的影响。方法：补肾方、柔肝方和化痰方含药血清培养早期和晚期滑膜细胞，MTS／PMS法测定OD值，hCOMP定量试剂盒测定COMP含量。结果：通过MTS／PMS法观察确定补肾方、柔肝方和化痰方含药血清培养滑膜细胞观察点在第5～7天；三方培养滑膜细胞OD值低于正常血清对照组（P≤0．05）。结论：补肾方、柔肝方与化痰方可以抑制体外培养早期和晚期膝骨关节炎患者滑膜细胞增殖，柔肝方可以降低体外培养滑膜细胞分泌液中COMP的浓度。
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| 关 键 词： | 滑膜细胞 骨关节炎 中药含药血清 软骨寡聚基质蛋白 COMP |
Effect of Invigorating Kidney Formula,Softening Liver Formula and Dissipating Phlegm Formula on Synoviocyte Proliferation and COMP Expression in Knee Osteoarthritis |
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| ZHANG Peng SHI Guantong ZHENG Yuxin CAO Yuelong Supported by National Nature Science Foundation.Effect of Invigorating Kidney Formula,Softening Liver Formula and Dissipating Phlegm Formula on Synoviocyte Proliferation and COMP Expression in Knee Osteoarthritis[J].Chinese Journal of Traditional Medical Traumatology & Orthopeics,2008,16(12):18-21. |
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| Authors: | ZHANG Peng SHI Guantong ZHENG Yuxin CAO Yuelong Supported by National Nature Science Foundation |
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| Institution: | ZHANG Peng1 SHI Guantong2 ZHENG Yuxin2 CAO Yuelong2 Supported by National Nature Science Foundation (NO. 30472222)1 Department of Acupuncture & manipulation , Orthopedics,Shanghai 6th hospital,Shanghai 200233,China2 Department of Orthopedics,Shuguang Hospital,Shanghai University of Traditional Chinese Medici |
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| Abstract: | Objective:To observe the effect of invigorating kidney formula (IKF),softening liver formula (SLF) and dissipating phlegm formula (DPF) on synoviocyte proliferation and cartilage oligomeric matrix protein (COMP) expression in knee osteoarthritis. Methods:Human synoviocytes were cultured in serum with IKF,SLF and DPF,respectively. MTS/PMS method was applied to measure OD value and hCOMP kits were used to measure COMP content. Results:The observation time of tissue culture was determined at 5-7day through the... |
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| Keywords: | COMP |
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