苦杏仁苷对大鼠肝星状细胞HSC—T6增殖和Bax基因表达的影响

目的：研究苦杏仁苷对大鼠肝星状细胞HSC—T6增殖的影响和对凋亡基因Bax的调控作用。方法：利用噻唑蓝染色法动态检测不同浓度的苦杏仁苷对大鼠HSC—T6细胞增殖的影响。利用PCR法检测苦杏仁苷对HSC—T6细胞凋亡基因Bax的表达水平。结果：苦杏仁各实验浓度组对HSC—T6细胞的增殖无直接抑制作用，但其高浓度10^－3mol／L和中浓度10^-4mol／L可以使HSC—T6细胞提前到达增殖高峰期；而各浓度组对HSC—T6细胞的Bax基因都有一定的诱导作用，并且存在量效关系。结论：苦杏仁苷不能直接抑制HSC—T6细胞的增殖，但可能通过诱导或上调凋亡基因Bax的过度表达，使细胞提前进入凋亡程序阶段，最终使活化的HSC—T6发生细胞凋亡，以起到抗肝纤维化的作用。

Effect of Amygdalin on Proliferation of Hepatic Stellate Cell HSC-T6 and Bax Gene Expression

Objective:To observe the effect of amygdalin on the proliferation of HSC-T6 and Bax gene expression.Methods:Proliferation of HSC-T6 cultured in the medium containing different concentrations of amygdalin was dynamically detected by methyl thiazolyl tetrazolium(MTT) assat.Bax gene expression level was detected by polymerase chain reaction(PCR) technique.Results:No direct inhibition effect was shown on the proliferation of HSC-T6 in all of amygdalin groups.But in amygdalin groups at 10-3 mol/L and 10-4mol/L,HSC-T6 reached the growth peak in advance.Amygdalin had certain effect on inducing Bax gene expression,and the effect was in dose-dependent manner.Conclusion:Amygdalin has no direct inhibition effect on proliferation of HSC-T6,but may induce or up-regulate Bax gene expression to promote apoptosis for anti-hepatic fibrosis.