首页 | 本学科首页   官方微博 | 高级检索  
检索        

散结镇痛胶囊中黄酮类成分的指纹图谱研究
引用本文:颜月园,萧伟,吴云,王伟,姜华,张怡,朱克近.散结镇痛胶囊中黄酮类成分的指纹图谱研究[J].中国实验方剂学杂志,2012,18(5):58-61.
作者姓名:颜月园  萧伟  吴云  王伟  姜华  张怡  朱克近
作者单位:1. 南京中医药大学,南京 210000;中药制药过程新技术国家重点实验室,江苏连云港222001
2. 中药制药过程新技术国家重点实验室,江苏连云港222001;江苏康缘药业股份有限公司,江苏连云港222001
基金项目:国家科技部重大新药创制项目(2011ZX09201-201-20)
摘    要:目的:采用高效液相色谱法测定散结镇痛胶囊中黄酮类成分的指纹图谱.方法:采用50%甲醇超声提取黄酮类成分进行分析检测.色谱条件为Waters Symmetry@ C18(4.6 mm×250 mm,5μm)色谱柱;流动相为乙腈-水,梯度洗脱;检测波长275 nm;流速1.0mL·min -1;柱温30℃.采用国家药典委员会出版的《中药色谱指纹图谱相似度评价》(2004年A版)软件,对10批不同批次的散结镇痛胶囊中黄酮类成分指纹图谱进行相似度计算.结果:指纹图谱中标记了15个共有峰,共有峰面积之和大于总峰面积的95%,利用指纹图谱相似度测试软件,测定10批散结镇痛胶囊中黄酮类成分指纹图谱相似度均在0.99以上.结论:方法具有良好的精密度、重复性、稳定性,各共有峰间分离度高,可用于散结镇痛胶囊质量的综合评价.

关 键 词:散结镇痛胶囊  黄酮类成分  指纹图谱  高效液相色谱法
收稿时间:2011/10/21 0:00:00

Fingerprint Analysis of Flavonoids in Sanjie Zhentong Capsules by HPLC
YAN Yue-yuan,XIAO Wei,WU Yun,WANG Wei,JIANG Hu,ZHANG Yi and ZHU Ke-jin.Fingerprint Analysis of Flavonoids in Sanjie Zhentong Capsules by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2012,18(5):58-61.
Authors:YAN Yue-yuan  XIAO Wei  WU Yun  WANG Wei  JIANG Hu  ZHANG Yi and ZHU Ke-jin
Institution:Nanjing University of Traditional Chinese Medicine, Nanjing 210000, China; State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China; Jangsu Kanion pharmaceutical Co., Ltd, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China; Jangsu Kanion pharmaceutical Co., Ltd, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China; Jangsu Kanion pharmaceutical Co., Ltd, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China; Jangsu Kanion pharmaceutical Co., Ltd, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China; Jangsu Kanion pharmaceutical Co., Ltd, Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China; Jangsu Kanion pharmaceutical Co., Ltd, Lianyungang 222001, China
Abstract:Objective:To establish the chromatographic fingerprint analysis for the quality control of the content of flavonoids in Sanjie Zhentong capsules.Method: Under the operation of ultrasonic extraction,the flavonoids from Sanjie Zhentong capsules were extracted in the 50% methanol.With Waters Symmetry@ C18(4.6 mm×250 mm,5 μm)column,and gradient elution was performed by mobile phase containing acetonitrile and water.The flow rate was 1.0 mL·min-1 and the detection wave length was at 203 nm.The temperature of column was maintained at 30 ℃.And data were analyzed by ’Similarity evaluation software for chromatographic Fingerprint of traditional Chinese medicine’ software to compare the quanlity of 11 batches of capsules samples.Result: Fifty common peaks were selected in chromatography,and all the common peaks were separated effectively.Conclusion: The accuracy,repeatability,and stability of this method were satisfied.The method developed can be used to evaluate the quality of Sanjie Zhentong capsules synthetically.
Keywords:Sanjie Zhentong capsules  flavonoids  fingerprint  HPLC
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《中国实验方剂学杂志》浏览原始摘要信息
点击此处可从《中国实验方剂学杂志》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号