半夏总生物碱对D-半乳糖所致衰老小鼠学习记忆障碍的改善作用

目的:研究半夏总生物碱(TAPT)对D-半乳糖诱导的衰老小鼠学习记忆的影响并初步探讨其作用机制。方法:昆明种小鼠100只,随机分为5组,即正常对照组,模型组,TAPT低、中、高剂量组(7.5,15,30 mg.kg-1),每组20只。模型组和TAPT组给予120 mg.kg-1的D-半乳糖注射液(sc,每天1次,连续8周),建立学习记忆障碍的衰老小鼠模型,对照组给予等量生理盐水;同时,TAPT各组给予对应浓度的TAPT药液(ig,每天1次,连续8周),对照组及模型组给予等量蒸馏水。采用跳台法和Y迷宫评价法测试TAPT对模型小鼠学习记忆障碍的改善作用,采用比色法测定小鼠脑组织中丙二醛(MDA)的含量,超氧化物歧化酶(SOD)和乙酰胆碱酯酶(AChE)的活性。结果:TAPT低、中、高剂量组小鼠Y迷宫训练时达标所需总次数减少,分别为(21.9±2.9),(20.8±3.2),(19.7±2.8)次;Y迷宫记忆测试时错误次数也明显减少,分别为(3.8±1.4),(3.2±1.7),(1.7±1.1)次;模型组小鼠脑组织SOD活性明显降低(28.98±13.08)U.mg-1,MDA及AChE含量明显增加(2.78±0.21)nmol.mg-1,(1.05±0.16)U.mg-1;经TAPT干预后,各剂量组小鼠脑组织中SOD活性增高(P<0.01),MDA含量明显降低(P<0.01);中、高剂量组AChE含量显著减少(P<0.01,P<0.05)。结论:应用TAPT干预D-半乳糖诱导的衰老小鼠,可明显改善其学习和记忆功能,这可能与其具有抗氧化作用及抑制AChE的活性有关。

Effects of Total Alkaloids from Pinellia ternate on Learning and Memory in Aging Mice Induced by D-Galactose

Objective:To study the effects of total alkaloids from Pinellia ternate (TAPT) on learning and memory ability in aging mice induced by D-galactose and its preliminary mechanism. Method: One hundred mice were randomly assigned into control group, model group, TAPT low, middle and high dose group(7.5, 15, 30 mg·kg^-1), 20 mice in each group. The TAPT and the model groups were given 120 mg·kg^-1 D-galactose (sc, for 8 weeks) to induce aging-mouse model, and the control group was given the same volume of saline water; meanwhile, the TAPT groups were given appropriate liquid (ig, for 8 weeks), while the control and the model groups were given the same volume of distilled water. The step-down test and the Y-maze test were adopted to reflect the learning and memory of mice; and the colorimetric method was used to determine the cellular content of malondialdehyde (MDA), the enzyme activity of superoxide dismutase (SOD) and acetylcholinesterase (AChE). Result: Compared to the model group, the TAPT treatment brought mice to shorten the time to reach the final in the Y-maze training at (21.9±2.9), (20.8±3.2) ,( 19.7±2.8) times respectively, and also reduce the mistakes in memory test at (3.8±1.4), (3.2±1.7),(1.7±1.1) times respectively. SOD activity in brain tissues of aging model mice reduced evidently at(28.98±13.08) U·mg^-1, while MDA and AChE contents increased evidently at (2.78±0.21)nmol·mg^-1 ,( 1.05±0.16)U·mg^-1, while TAPT treatment could evidently decrease MDA(P<0.01) and AChE contents(P<0.01),and increase SOD activity(P<0.01). Conclusion: TAPT treatment could help aging model mice induced by D-galactose evidently to enhance their learning and memory, which might be related to its antioxidation and its inhibitory activity to AChE.